HSP60 Polyclonal antibody

HSP60 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15282-1-AP

Synonyms

HSP 60, HSP60, HSP65, HSPD1



Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, NIH/3T3 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:8000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

15282-1-AP targets HSP60 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity chick, chicken, fish, human, mouse, rat, rice, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSP60 fusion protein Ag7401
Full Name heat shock 60kDa protein 1 (chaperonin)
Calculated molecular weight 61 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC003030
Gene symbol HSPD1
Gene ID (NCBI) 3329
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSPD1, also known as HSP60, belongs to the chaperonin family and acts as a chaperone to enhance cell survival under physiological stresses. Hsp60 has been shown to be connected with many aspects of cell functions such as protein folding and assembling of polypeptide chains in mitochondria. Recently it has been reported that HSP60 is associated with apoptosis or inhibition of cancer cell growth. (21822415)

Protocols

Product Specific Protocols
WB protocol for HSP60 antibody 15282-1-APDownload protocol
IHC protocol for HSP60 antibody 15282-1-APDownload protocol
IF protocol for HSP60 antibody 15282-1-APDownload protocol
IP protocol for HSP60 antibody 15282-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

EBioMedicine

Targeting glutaminase 1 attenuates stemness properties in hepatocellular carcinoma by increasing reactive oxygen species and suppressing Wnt/beta-catenin pathway.

Authors - Binghua Li
humanWB

Am J Transl Res

Fetal bovine serum inhibits neomycin-induced apoptosis of hair cell-like HEI-OC-1 cells by maintaining mitochondrial function.

Authors - Ming Guan
ratWB,IHC

Neuropeptides

Protective effects of ghrelin on brain mitochondria after cardiac arrest and resuscitation.

Authors - Hongying Xu
chickenWB

Environ Sci Pollut Res Int

Arsenic and/or copper caused inflammatory response via activation of inducible nitric oxide synthase pathway and triggered heat shock protein responses in testis tissues of chicken.

Authors - Yizhi Shao
chickenWB

Int Immunopharmacol

Impacts of simultaneous exposure to arsenic (III) and copper (II) on inflammatory response, immune homeostasis, and heat shock response in chicken thymus.

Authors - Juanjuan Liu
humanWB

JCI Insight

Extensive skeletal muscle cell mitochondriopathy distinguishes critical limb ischemia patients from claudicants.

Authors - Terence E Ryan