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  • KD/KO Validated

IFITM3 Polyclonal antibody

IFITM3 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 11714-1-AP

Synonyms

1 8U, Fragilis, IFITM3, IP15



Tested Applications

Positive WB detected inHeLa cells, mouse colon tissue, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11714-1-AP targets IFITM3 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity goat, human, mouse, rat, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFITM3 fusion protein Ag2285
Full Name interferon induced transmembrane protein 3 (1-8U)
Calculated molecular weight 133 aa, 15 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC006794
Gene symbol IFITM3
Gene ID (NCBI) 10410
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFITM3, also named as interferon-inducible protein 1-8U, belongs to the CD225 family. It is IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus, by inhibiting the early steps of replication. IFITM3 is identified as interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. IFITM3, the most potent antiviral IFITM, was found to inhibit an uncharacterized early infectious event after VSV endocytosis, but before primary transcription of its viral genome. IFITM proteins are viral restriction factors that can inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. They differentially restrict the entry of a broad range of enveloped viruses, and modulate cellular tropism independently of viral receptor expression. Catalog#11714-1-AP is a rabbit polyclonal antibody raised against the full-length of human IFITM3.

Protocols

Product Specific Protocols
WB protocol for IFITM3 antibody 11714-1-APDownload protocol
IHC protocol for IFITM3 antibody 11714-1-APDownload protocol
IF protocol for IFITM3 antibody 11714-1-APDownload protocol
IP protocol for IFITM3 antibody 11714-1-APDownload protocol
FC protocol for IFITM3 antibody 11714-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Nat Chem Biol

IFITM3 directly engages and shuttles incoming virus particles to lysosomes.

Authors - Jennifer S Spence
  • KO Validated
humanWB

Int J Biol Macromol

Construction, expression and antiviral activity analysis of recombinant adenovirus expressing human IFITM3 in vitro.

Authors - Shouwen Du
humanWB

MBio

Vpr Enhances HIV-1 Env Processing and Virion Infectivity in Macrophages by Modulating TET2-Dependent IFITM3 Expression.

Authors - Qi Wang
  • KD Validated
mouseIHC

Cell Rep

Cell Lineage Tracing Identifies Hormone-Regulated and Wnt-Responsive Vaginal Epithelial Stem Cells.

Authors - Ayesha Ali
humanWB

Cell Chem Biol

Site-Specific Photo-Crosslinking Proteomics Reveal Regulation of IFITM3 Trafficking and Turnover by VCP/p97 ATPase.

Authors - Xiaojun Wu
  • KO Validated
goatWB

Res Vet Sci

Infection of goats with goatpox virus triggers host antiviral defense through activation of innate immune signaling.

Authors - Xiancheng Zeng