IL-22RA2 Monoclonal antibody

IL-22RA2 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

5G11A9

Cat no : 66190-1-Ig

Synonyms

CRF2 10, CRF2 S1, CRF2X, IL 22 receptor subunit alpha 2, IL 22BP, IL 22R alpha 2, IL 22RA2, IL22BP, IL22RA2, IL-22RA2, Interleukin 22 binding protein, ZcytoR16



Tested Applications

Positive WB detected inRaji cells
Positive IHC detected inhuman small intestine tissue, human breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman breast cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66190-1-Ig targets IL-22RA2 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen IL-22RA2 fusion protein Ag18682
Full Name interleukin 22 receptor, alpha 2
Calculated Molecular Weight 263 aa, 31 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC125167
Gene Symbol IL22RA2
Gene ID (NCBI) 116379
RRIDAB_2881585
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-22 receptor subunit alpha-2 (IL22RA2) is a soluble member of the type II cytokine receptor family. Through binding to IL22, IL22RA2 prevents the interaction of IL22 with the functional IL22-R complex and blocks the activity of IL22 (in vitro). IL22RA2 may play an important role as an IL22 antagonist in the regulation of inflammatory responses. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Protocols

Product Specific Protocols
WB protocol for IL-22RA2 antibody 66190-1-IgDownload protocol
IHC protocol for IL-22RA2 antibody 66190-1-IgDownload protocol
IF protocol for IL-22RA2 antibody 66190-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB,IHC

Front Cell Infect Microbiol

Oral Administration of Lactobacillus rhamnosus GG Ameliorates Salmonella Infantis-Induced Inflammation in a Pig Model via Activation of the IL-22BP/IL-22/STAT3 Pathway.

Authors - Gui-Yan Yang
humanFC

Cells

The Rare IL22RA2 Signal Peptide Coding Variant rs28385692 Decreases Secretion of IL-22BP Isoform-1, -2 and -3 and Is Associated with Risk for Multiple Sclerosis.

Authors - Paloma Gómez-Fernández
humanWB,IF

Front Immunol

Long Interleukin-22 Binding Protein Isoform-1 Is an Intracellular Activator of the Unfolded Protein Response.

Authors - Paloma Gómez-Fernández