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  • KD/KO Validated

INPP5E Polyclonal antibody

INPP5E Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17797-1-AP

Synonyms

INPP5E, PPI5PIV



Tested Applications

Positive WB detected inSH-SY5Y cells, HEK-293 cells, mouse brain tissue, mouse testis tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman testis tissue, human brain tissue, human heart tissue, human kidney tissue, human ovary tissue, human placenta tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

17797-1-AP targets INPP5E in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivity human, mouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen INPP5E fusion protein Ag11959
Full Name inositol polyphosphate-5-phosphatase, 72 kDa
Calculated molecular weight 644 aa, 70 kDa
Observed molecular weight 64-66 kDa
GenBank accession numberBC028032
Gene symbol INPP5E
Gene ID (NCBI) 56623
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

INPP5E(72 kDa inositol polyphosphate 5-phosphatase) converts phosphatidylinositol 3,4,5-trisphosphate (PtdIns 3,4,5-P3) to PtdIns-P2.In mouse, highest protein expression was in brain, heart, and testis, with lower expression in thymus and lung, and very little expression in kidney, spleen, and liver(PMID:10764818). Defects in INPP5E are the cause of Joubert syndrome type 1 (JBTS1) and mental retardation-truncal obesity-retinal dystrophy-micropenis (MORMS)(PMID:19668215). It has 2 isoforms with the molecular mass of 66 kDa and 70 kDa.

Protocols

Product Specific Protocols
WB protocol for INPP5E antibody 17797-1-APDownload protocol
IHC protocol for INPP5E antibody 17797-1-APDownload protocol
IP protocol for INPP5E antibody 17797-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Biochem Biophys Res Commun

TULP3 is required for localization of membrane-associated proteins ARL13B and INPP5E to primary cilia.

Authors - Sarina Han
humanWB,IF

Proc Natl Acad Sci U S A

Disruption of RPGR protein interaction network is the common feature of RPGR missense variations that cause XLRP.

Authors - Qihong Zhang
  • KO Validated
mouseIF

Curr Biol

Tulp3 Regulates Renal Cystogenesis by Trafficking of Cystoproteins to Cilia.

Authors - Sun-Hee Hwang
mouseIF

Development

The ciliary GTPase Arl3 maintains tissue architecture by directing planar spindle orientation during epidermal morphogenesis.

Authors - Samip R Bhattarai
humanWB

Hum Gene Ther

Development of a Molecularly Stable Gene Therapy Vector for the Treatment of RPGR-associated X-linked Retinitis Pigmentosa.

Authors - Joseph C Giacalone
humanIF

J Cell Sci

Regulation of ciliary retrograde protein trafficking by Joubert syndrome proteins ARL13B and INPP5E.

Authors - Shohei Nozaki