|Positive WB detected in||SH-SY5Y cells, HEK-293 cells, mouse brain tissue, mouse testis tissue|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human testis tissue, human brain tissue, human heart tissue, human kidney tissue, human ovary tissue, human placenta tissue, human spleen tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||hTERT-RPE1 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
17797-1-AP targets INPP5E in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, rat, mouse samples.
|Tested Reactivity||human, rat, mouse|
|Cited Reactivity||human, mouse, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||INPP5E fusion protein Ag11959|
|Full Name||inositol polyphosphate-5-phosphatase, 72 kDa|
|Calculated molecular weight||644 aa, 70 kDa|
|Observed molecular weight||64-66 kDa|
|GenBank accession number||BC028032|
|Gene ID (NCBI)||56623|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
INPP5E(72 kDa inositol polyphosphate 5-phosphatase) converts phosphatidylinositol 3,4,5-trisphosphate (PtdIns 3,4,5-P3) to PtdIns-P2.In mouse, highest protein expression was in brain, heart, and testis, with lower expression in thymus and lung, and very little expression in kidney, spleen, and liver(PMID:10764818). Defects in INPP5E are the cause of Joubert syndrome type 1 (JBTS1) and mental retardation-truncal obesity-retinal dystrophy-micropenis (MORMS)(PMID:19668215). It has 2 isoforms with the molecular mass of 66 kDa and 70 kDa.
Biochem Biophys Res Commun
TULP3 is required for localization of membrane-associated proteins ARL13B and INPP5E to primary cilia.
Proc Natl Acad Sci U S A
Disruption of RPGR protein interaction network is the common feature of RPGR missense variations that cause XLRP.
Tulp3 Regulates Renal Cystogenesis by Trafficking of Cystoproteins to Cilia.
The ciliary GTPase Arl3 maintains tissue architecture by directing planar spindle orientation during epidermal morphogenesis.
Hum Gene Ther
Development of a Molecularly Stable Gene Therapy Vector for the Treatment of RPGR-associated X-linked Retinitis Pigmentosa.
J Cell Sci
Regulation of ciliary retrograde protein trafficking by Joubert syndrome proteins ARL13B and INPP5E.