• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

IRF5 Polyclonal antibody

IRF5 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10547-1-AP

Synonyms

interferon regulatory factor 5, IRF 5, IRF5, SLEB10

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using IRF5 Polyclonal antibody (10547-1-AP)

    WB analysis using 10547-1-AP

    THP-1 cells were subjected to SDS PAGE followed by western blot with 10547-1-AP (IRF5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using IRF5 Polyclonal antibody (10547-1-AP)

    WB analysis of Jurkat using 10547-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10547-1-AP (IRF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using IRF5 Polyclonal antibody (10547-1-AP)

    WB analysis of Jurkat using 10547-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10547-1-AP (IRF5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using IRF5 Polyclonal antibody (10547-1-AP)

    WB analysis of SH-SY5Y using 10547-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10547-1-AP (IRF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using IRF5 Polyclonal antibody (10547-1-AP)

    IP experiment of HeLa using 10547-1-AP

    IP result of anti-IRF5 (IP:10547-1-AP, 4ug; Detection:10547-1-AP 1:300) with HeLa cells lysate 1600ug.

  • Immunoprecipitation (IP) experiment of HeLa cells using IRF5 Polyclonal antibody (10547-1-AP)

    IP experiment of HeLa using 10547-1-AP

    IP result of anti-IRF5 (IP:10547-1-AP, 4ug; Detection:10547-1-AP 1:300) with HeLa cells lysate 1600ug.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using IRF5 Polyclonal antibody (10547-1-AP)

    IHC staining of human prostate cancer using 10547-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10547-1-AP (IRF5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using IRF5 Polyclonal antibody (10547-1-AP)

    IHC staining of human breast cancer using 10547-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10547-1-AP (IRF5 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using IRF5 Polyclonal antibody (10547-1-AP)

    IHC staining of human breast cancer using 10547-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10547-1-AP (IRF5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using IRF5 Polyclonal antibody (10547-1-AP)

    IHC staining of human breast cancer using 10547-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10547-1-AP (IRF5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using IRF5 Polyclonal antibody (10547-1-AP)

    IHC staining of human prostate cancer using 10547-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10547-1-AP (IRF5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using IRF5 Polyclonal antibody (10547-1-AP)

    IF Staining of HepG2 using 10547-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IRF5 antibody (10547-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using IRF5 Polyclonal antibody (10547-1-AP)

    IF Staining of HepG2 using 10547-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IRF5 antibody (10547-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using IRF5 Polyclonal antibody (10547-1-AP)

    IF Staining of HepG2 using 10547-1-AP

    Immunofluorescent analysis of HepG2 cells using 10547-1-AP (IRF5 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HepG2 cells using IRF5 Polyclonal antibody (10547-1-AP)

    FC experiment of HepG2 using 10547-1-AP

    1X10^6 HepG2 cells were stained with 0.2ug IRF5 antibody (10547-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Tested Applications

Positive WB detected inTHP-1 cells, SH-SY5Y cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10547-1-AP targets IRF5 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, brovine, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IRF5 fusion protein Ag0841
Full Name interferon regulatory factor 5
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC004201
Gene Symbol IRF5
Gene ID (NCBI) 3663
RRIDAB_2280474
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IRF5, also named as SLEB10, contians one IRF tryptophan pentad repeat DNA-binding domain and belongs to the IRF family. It is a transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. It is activated by TLR7 or TLR8 signaling. Genetic variations in IRF5 are associated with susceptibility to inflammatory bowel disease type 14 (IBD14) and systemic lupus erythematosus type 10 (SLEB10). Alternative splice variant encoding different isoforms exist. Catalog#10547-1-AP is a rabbit polyclonal antibody raised against the C-terminal of human IRF5.

Protocols

Product Specific Protocols
WB protocol for IRF5 antibody 10547-1-APDownload protocol
IHC protocol for IRF5 antibody 10547-1-APDownload protocol
IF protocol for IRF5 antibody 10547-1-APDownload protocol
IP protocol for IRF5 antibody 10547-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Br J Sports Med

Chronic inflammation is a feature of Achilles tendinopathy and rupture.

Authors - Stephanie Georgina Dakin
View Article
mouseWB

J Immunother Cancer

Ccl3 enhances docetaxel chemosensitivity in breast cancer by triggering proinflammatory macrophage polarization.

Authors - Dandan Sheng
View Article
ratWB

Am J Transplant

Characterization of Transfusion-Elicited Acute Antibody-Mediated Rejection in a Rat Model of Kidney Transplantation.

Authors - G Huang
View Article
mouseWB

J Control Release

PEGylation of interleukin-10 improves the pharmacokinetic profile and enhances the antifibrotic effectivity in CCl?-induced fibrogenesis in mice.

Authors - Mattos Adriana A
View Article
mouseIHC

Br J Pharmacol

Ibuprofen and diclofenac treatments reduce proliferation of pancreatic acinar cells upon inflammatory injury and mitogenic stimulation.

Authors - Marta Bombardo
View Article
mouseWB,IF

Biochem Pharmacol

Quercetin ameliorates kidney injury and fibrosis by modulating M1/M2 macrophage polarization.

Authors - Hong Lu
View Article