|Positive WB detected in||Jurkat cells, A549 cells, HeLa cells, human heart tissue, NIH/3T3 cells, SH-SY5Y cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human prostate cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10547-1-AP targets IRF5 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, sheep|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IRF5 fusion protein Ag0841|
|Full Name||interferon regulatory factor 5|
|Calculated molecular weight||56 kDa|
|Observed molecular weight||56 kDa|
|GenBank accession number||BC004201|
|Gene ID (NCBI)||3663|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
IRF5, also named as SLEB10, contians one IRF tryptophan pentad repeat DNA-binding domain and belongs to the IRF family. It is a transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. It is activated by TLR7 or TLR8 signaling. Genetic variations in IRF5 are associated with susceptibility to inflammatory bowel disease type 14 (IBD14) and systemic lupus erythematosus type 10 (SLEB10). Alternative splice variant encoding different isoforms exist. Catalog#10547-1-AP is a rabbit polyclonal antibody raised against the C-terminal of human IRF5.
|Product Specific Protocols|
|WB protocol for IRF5 antibody 10547-1-AP||Download protocol|
|IHC protocol for IRF5 antibody 10547-1-AP||Download protocol|
|IF protocol for IRF5 antibody 10547-1-AP||Download protocol|
|IP protocol for IRF5 antibody 10547-1-AP||Download protocol|
|FC protocol for IRF5 antibody 10547-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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