Integrin Alpha 6 Polyclonal antibody

Integrin Alpha 6 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 27189-1-AP

Synonyms

CD49f, Integrin alpha 6, Integrin alpha-6, integrin, alpha 6, ITGA6, ITGA6B, VLA 6



Tested Applications

Positive WB detected inA549 cells, A431 cells
Positive IHC detected inhuman kidney tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 27189-1-AP is Integrin Alpha 6 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity Hamster, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Integrin Alpha 6 fusion protein Ag25835
Full Name integrin, alpha 6
Calculated molecular weight 1073 aa, 119 kDa
Observed molecular weight 110-130 kDa
GenBank accession numberBC136456
Gene symbol ITGA6
Gene ID (NCBI) 3655
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Integrin Alpha 6 antibody 27189-1-APDownload protocol
IHC protocol for Integrin Alpha 6 antibody 27189-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Neoplasia

NFAT1 Hypermethylation Promotes Epithelial-Mesenchymal Transition and Metastasis in Nasopharyngeal Carcinoma by Activating ITGA6 Transcription.

Authors - Jian Zhang
humanWB,IHC

Am J Transl Res

MiR-302a-5p suppresses cell proliferation and invasion in non-small cell lung carcinoma by targeting ITGA6.

Authors - Wei Chen
humanWB

Biochem Biophys Res Commun

MicroRNA-377-3p alleviates IL-1β-caused chondrocyte apoptosis and cartilage degradation in osteoarthritis in part by downregulating ITGA6.

Authors - Yihui Tu
HamsterIF

J Virol

Single-cell analysis of the impact of host-cell heterogeneity on infection with foot and mouth disease virus.

Authors - Xiu Xin