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  • Featured Product
  • KD/KO Validated

KLHL14-Specific Polyclonal antibody

KLHL14-Specific Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14849-1-AP

Synonyms

kelch like 14 (Drosophila), Kelch like protein 14, KIAA1384, KLHL14, KLHL14-Specific

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using KLHL14-Specific Polyclonal antibody (14849-1-AP)

    WB analysis of HeLa using 14849-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using KLHL14-Specific Polyclonal antibody (14849-1-AP)

    WB analysis using 14849-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using KLHL14-Specific Polyclonal antibody (14849-1-AP)

    WB analysis of rat brain using 14849-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using KLHL14-Specific Polyclonal antibody (14849-1-AP)

    WB analysis of HeLa using 14849-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using KLHL14-Specific Polyclonal antibody (14849-1-AP)

    WB analysis of mouse heart using 14849-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using KLHL14-Specific Polyclonal antibody (14849-1-AP)

    WB analysis of HepG2 using 14849-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using KLHL14-Specific Polyclonal antibody (14849-1-AP)

    WB analysis of mouse testis using 14849-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using KLHL14-Specific Polyclonal antibody (14849-1-AP)

    WB analysis of mouse lung using 14849-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using KLHL14-Specific Polyclonal antibody (14849-1-AP)

    WB analysis of mouse liver using 14849-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using KLHL14-Specific Polyclonal antibody (14849-1-AP)

    WB analysis of SH-SY5Y using 14849-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using KLHL14-Specific Polyclonal antibody (14849-1-AP)

    WB analysis of mouse brain using 14849-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using KLHL14-Specific Polyclonal antibody (14849-1-AP)

    WB analysis of mouse ovary using 14849-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using KLHL14-Specific Polyclonal antibody (14849-1-AP)

    WB analysis of SH-SY5Y using 14849-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14849-1-AP (KLHL14-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using KLHL14-Specific Polyclonal antibody (14849-1-AP)

    IHC staining of human cervical cancer using 14849-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14849-1-AP (KLHL14-Specific Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using KLHL14-Specific Polyclonal antibody (14849-1-AP)

    IHC staining of human heart using 14849-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14849-1-AP (KLHL14-Specific Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using KLHL14-Specific Polyclonal antibody (14849-1-AP)

    IHC staining of human heart using 14849-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14849-1-AP (KLHL14-Specific Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using KLHL14-Specific Polyclonal antibody (14849-1-AP)

    IHC staining of human cervical cancer using 14849-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14849-1-AP (KLHL14-Specific Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KLHL14-Specific Polyclonal antibody (14849-1-AP)

    IF Staining of HepG2 using 14849-1-AP

    Immunofluorescent analysis of HepG2 cells, using KLHL14 antibody 14849-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inHeLa cells, mouse heart tissue, mouse testis tissue, mouse lung tissue, mouse liver tissue, SH-SY5Y cells, mouse brain tissue, mouse ovary tissue, HepG2 cells, rat brain tissue
Positive IHC detected inhuman heart tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14849-1-AP targets KLHL14-Specific in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name kelch-like 14 (Drosophila)
Calculated Molecular Weight 70 kDa
Observed Molecular Weight39-45 kDa
GenBank Accession NumberNM_020805
Gene Symbol KLHL14
Gene ID (NCBI) 57565
RRIDAB_2131171
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KLHL14, a member of the Kelch-like gene family, has six Kelch domains and is localized to the endoplasmic reticulum. KLHL14, a novel tumor suppressor, is a subunit of a ubiquitin ligase that associates with the endoplasmic reticulum-associated protein degradation (ERAD) machinery, regulating of B cell receptor-dependent NF-κB signaling (PMID: 32127472). KLHL14 has two isoforms of 70kDa and 43kDa.

Protocols

Product Specific Protocols
WB protocol for KLHL14-Specific antibody 14849-1-APDownload protocol
IHC protocol for KLHL14-Specific antibody 14849-1-APDownload protocol
IF protocol for KLHL14-Specific antibody 14849-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Clin Exp Pathol

Kelch-like protein 14 promotes proliferation and migration of ovarian cancer cells.

Authors - Zheng Chen
  • KD Validated
View Article