KRAS-2A-specific Polyclonal antibody

KRAS-2A-specific Polyclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 16156-1-AP

Synonyms

C K RAS, c Ki ras, GTPase KRas, K Ras 2, K RAS2A, K RAS2B, K RAS4A, K RAS4B, KI RAS, KRAS, KRAS1, KRAS2, KRAS2A, KRAS2B speicifc, KRAS4A, Krasa, Krasb, NS3, RASK2



Tested Applications

Positive WB detected inSH-SY5Y cells
Positive IF detected inSH-SY5Y cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16156-1-AP targets KRAS-2A-specific in WB, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Calculated molecular weight 21 kDa
Observed molecular weight 21-24 kDa
GenBank accession numberNM_033360
Gene symbol KRAS
Gene ID (NCBI) 3845
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAS (derived from 'RAt Sarcoma' virus) gene family includes KRAS, HRAS, and NRAS. These genes encode very similar 21 kDa proteins and are homologs of rodent sarcoma virus genes that have transforming abilities. KRAS has two isoforms, 2A and 2B. This antibody is specific for KRAS-2A, which also named as KRAS-4A .

Protocols

Product Specific Protocols
WB protocol for KRAS-2A-specific antibody 16156-1-APDownload protocol
IF protocol for KRAS-2A-specific antibody 16156-1-APDownload protocol
FC protocol for KRAS-2A-specific antibody 16156-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols