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Cytokeratin 15 Monoclonal antibody

Cytokeratin 15 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

6B4F8

Cat no : 60247-1-Ig

Synonyms

CK 15, CK15, Cytokeratin 15, K15, K1CO, keratin 15, KRT15, KRTB

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using Cytokeratin 15 Monoclonal antibody (60247-1-Ig)

    WB analysis of A431 using 60247-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 60247-1-Ig (Cytokeratin 15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Cytokeratin 15 Monoclonal antibody (60247-1-Ig)

    IHC staining of human oesophagus cancer using 60247-1-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60247-1-Ig (Cytokeratin 15 antibody at dilution of 1:500 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Cytokeratin 15 Monoclonal antibody (60247-1-Ig)

    IHC staining of human oesophagus cancer using 60247-1-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60247-1-Ig (Cytokeratin 15 antibody at dilution of 1:500 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Cytokeratin 15 Monoclonal antibody (60247-1-Ig)

    IHC staining of human skin cancer using 60247-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60247-1-Ig (Cytokeratin 15 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Cytokeratin 15 Monoclonal antibody (60247-1-Ig)

    IHC staining of human skin cancer using 60247-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60247-1-Ig (Cytokeratin 15 antibody at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Cytokeratin 15 Monoclonal antibody (60247-1-Ig)

    IF Staining of HeLa using 60247-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cytokeratin 15 antibody (60247-1-Ig, Clone: 6B4F8 ) at dilution of 1:25 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of A431 cells using Cytokeratin 15 Monoclonal antibody (60247-1-Ig)

    FC experiment of A431 using 60247-1-Ig

    1X10^6 A431 cells were intracellularly stained with 0.2 ug Anti-Human Cytokeratin 15 (60247-1-Ig, Clone:6B4F8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

Tested Applications

Positive WB detected inA431 cells
Positive IHC detected inhuman oesophagus cancer tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

The immunogen of 60247-1-Ig is Cytokeratin 15 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cytokeratin 15 fusion protein Ag0185
Full Name keratin 15
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC002641
Gene Symbol KRT15
Gene ID (NCBI) 3866
RRIDAB_2881369
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 15 is a type I cytokeratin. It is found in some progenitor basal cells within complex epithelia.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 15 antibody 60247-1-IgDownload protocol
IHC protocol for Cytokeratin 15 antibody 60247-1-IgDownload protocol
IF protocol for Cytokeratin 15 antibody 60247-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Oxid Med Cell Longev

Exosomes Derived from Dermal Papilla Cells Mediate Hair Follicle Stem Cell Proliferation through the Wnt3a/β-Catenin Signaling Pathway

Authors - Jiali Li
View Article