Filter: AllWBSandwich ELISAIHCIF/ICCCytometric bead array

WB analysis of A431 using 60247-1-Ig (same clone as 60247-1-PBS)
A431 cells were subjected to SDS PAGE followed by western blot with 60247-1-Ig (Cytokeratin 15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60247-1-PBS in a different storage buffer formulation.
Sandwich ELISA standard curve of MP50002-1
Sandwich ELISA standard curve of MP50002-1, KRT15/Cytokeratin 15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60247-1-PBS. Detection antibody: HRP-conjugated 60247-2-PBS. Standard: Ag0185. Range: 0.313-20 ng/mL.
IHC staining of human oesophagus cancer using 60247-1-Ig (same clone as 60247-1-PBS)
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60247-1-Ig (Cytokeratin 15 antibody at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 60247-1-PBS in a different storage buffer formulation.
IHC staining of human oesophagus cancer using 60247-1-Ig (same clone as 60247-1-PBS)
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60247-1-Ig (Cytokeratin 15 antibody at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 60247-1-PBS in a different storage buffer formulation.
IHC staining of human skin cancer using 60247-1-Ig (same clone as 60247-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60247-1-Ig (Cytokeratin 15 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60247-1-PBS in a different storage buffer formulation.
IHC staining of human skin cancer using 60247-1-Ig (same clone as 60247-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60247-1-Ig (Cytokeratin 15 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60247-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 60247-1-Ig (same clone as 60247-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cytokeratin 15 antibody (60247-1-Ig, Clone: 6B4F8 ) at dilution of 1:25 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60247-1-PBS in a different storage buffer formulation.
Cytometric bead array standard curve of MP50002-1
Cytometric bead array standard curve of MP50002-1, KRT15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60247-1-PBS. Detection antibody: 60247-2-PBS. Standard: Ag0185. Range: 1.563-100 ng/mL.

Cytokeratin 15 Monoclonal antibody, PBS Only (Capture)

Cytokeratin 15 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Cat No. 60247-1-PBS

Clone No.6B4F8

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

KRT15, 6B4F8, CK 15, CK15, CK-15

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

60247-1-PBS targets Cytokeratin 15 as part of a matched antibody pair:

MP50002-1: 60247-1-PBS capture and 60247-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, mouse
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag0185 Product name: Recombinant human KRT15 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 238-450 aa of BC002641 Sequence: AYLKKNHEEEMKEFSSQLAGQVNVEMDAAPGVDLTRVLAEMREQYEAMAEKNRRDVEAWFFSKTEELNKEVASNTEMIQTSKTEITDLRRTMQELEIELQSQLSMKAGLENSLAETECRYATQLQQIQGLIGGLEAQLSELRCEMEAQNQEYKMLLDIKTRLEQEIATYRSLLEGQDAKMAGIGIREASSGGGGSSSNFHINVEESVDGQVVS Predict reactive species
Full Name	keratin 15
Calculated Molecular Weight	49 kDa
Observed Molecular Weight	49 kDa
GenBank Accession Number	BC002641
Gene Symbol	KRT15
Gene ID (NCBI)	3866
RRID	AB_2881369
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P19012
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 15 is a type I cytokeratin. It is found in some progenitor basal cells within complex epithelia.