Lysates of various cell lines and tissues were subjected to SDS PAGE followed by western blot with 12987-1-AP (Lamin B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours

Various lysates were subjected to SDS PAGE followed by western blot with 12987-1-AP (Lamin B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours

HepG2 cells were subjected to SDS PAGE followed by western blot with 12987-1-AP(LMNB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

K-562 cells were subjected to SDS PAGE followed by western blot with 12987-1-AP(LMNB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

mouse eye tissue were subjected to SDS PAGE followed by western blot with 12987-1-AP(LMNB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12987-1-AP(LMNB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

mouse lung tissue were subjected to SDS PAGE followed by western blot with 12987-1-AP (Lamin B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

mouse lung tissue were subjected to SDS PAGE followed by western blot with 12987-1-AP(LMNB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12987-1-AP(LMNB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

mouse spleen tissue were subjected to SDS PAGE followed by western blot with 12987-1-AP(LMNB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12987-1-AP(LMNB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 12987-1-AP(LMNB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

U-937 cells were subjected to SDS PAGE followed by western blot with 12987-1-AP(LMNB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

Y79 cells were subjected to SDS PAGE followed by western blot with 12987-1-AP(LMNB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Immunohistochemical analysis of paraffin-embedded human breast cancer using 12987-1-AP(LMNB1 antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical analysis of paraffin-embedded human kidney slide using 12987-1-AP( LMNB1 Antibody) at dilution of 1:50

Immunohistochemical analysis of paraffin-embedded human kidney slide using 12987-1-AP( LMNB1 Antibody) at dilution of 1:50

IP Result of anti-LMNB1 (IP:12987-1-AP, 3ug; Detection:12987-1-AP 1:1000) with HeLa cells lysate 2500ug.

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HepG2 cells using 12987-1-AP(Lamin B1 antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunofluorescent analysis of HepG2 cells, using LMNB1 antibody 12987-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Immunofluorescent analysis of ( 4% PFA) fixed human skin cancer tissue using 12987-1-AP (Lamin B1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunofluorescent analysis of ( -20°C Ethanol) fixed NIH/3T3 cells using 12987-1-AP (Lamin B1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

1X10^6 HEK-293 cells were stained with 0.2ug Lamin B1 antibody (12987-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IgG with dilution 1:100.