Lamin B1 Polyclonal antibody

Name: Lamin B1 Rabbit Polyclonal antibody
Brand: Proteintech
SKU: 12987-1-AP
Rating: 5.00 (7 reviews)

Lamin B1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IP, IHC, IF, FC, ChIP, ELISA

Conjugate

Unconjugated

Publications

813

Reviews (7)

Cat no : 12987-1-AP

Synonyms

ADLD, lamin B1, LMN, LMN2, LMNB, LMNB1

Validation Data Gallery
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WB analysis using 12987-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12987-1-AP (Lamin B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis using 12987-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12987-1-AP (Lamin B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 12987-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 12987-1-AP (Lamin B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 12987-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12987-1-AP (Lamin B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of U-937 using 12987-1-AP
U-937 cells were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse spleen using 12987-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 12987-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 12987-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 12987-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Y79 using 12987-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse eye using 12987-1-AP
mouse eye tissue were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 12987-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 12987-1-AP
IP Result of anti-LMNB1 (IP:12987-1-AP, 3ug; Detection:12987-1-AP 1:1000) with HeLa cells lysate 2500ug.
IHC staining of human liver cancer using 12987-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 12987-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney slide using 12987-1-AP (LMNB1 Antibody) at dilution of 1:50.
IHC staining of human kidney using 12987-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney slide using 12987-1-AP (LMNB1 Antibody) at dilution of 1:50.
IHC staining of human colon cancer using 12987-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 12987-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 12987-1-AP (Lamin B1 antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 12987-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Lamin B1 antibody (12987-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HepG2 using 12987-1-AP
Immunofluorescent analysis of HepG2 cells, using LMNB1 antibody 12987-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IF Staining of NIH/3T3 using 12987-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using 12987-1-AP (Lamin B1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of human skin cancer using 12987-1-AP
Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using 12987-1-AP (Lamin B1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HEK-293 using 12987-1-AP
1X10^6 HEK-293 cells were stained with 0.2ug Lamin B1 antibody (12987-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IgG with dilution 1:100.

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Cat No. 12987-1-AP

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Tested Applications

Positive WB detected in	HeLa cells, U-937 cells, mouse spleen tissue, K-562 cells, Y79 cells, mouse pancreas tissue, SH-SY5Y cells, mouse kidney tissue, mouse lung tissue, mouse eye tissue, HepG2 cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human liver cancer tissue, human colon cancer tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HepG2 cells, human skin cancer tissue, NIH/3T3 cells
Positive FC detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 2 publications below
WB	See 762 publications below
IHC	See 4 publications below
IF	See 42 publications below
FC	See 1 publications below
ChIP	See 2 publications below

Product Information

12987-1-AP targets Lamin B1 in WB, IP, IHC, IF, FC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, Bombyx mori, canine, chicken, hamster, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Lamin B1 fusion protein Ag3631
Full Name	lamin B1
Calculated molecular weight	66 kDa
Observed molecular weight	66-70 kDa
GenBank accession number	BC012295
Gene symbol	LMNB1
Gene ID (NCBI)	4001
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Expression of uncleavable mutant lamin A or B caused significant delays in the onset of chromatin condensation and nuclear shrinkage during apoptosis (PMID:11953316). This protein is not suitable for samples where the nuclear envelope has been removed.

Protocols

Product Specific Protocols
WB protocol for Lamin B1 antibody 12987-1-AP	Download protocol
IHC protocol for Lamin B1 antibody 12987-1-AP	Download protocol
IF protocol for Lamin B1 antibody 12987-1-AP	Download protocol
IP protocol for Lamin B1 antibody 12987-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	Nat Biotechnol Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases Authors - Matthew T N Yarnall View Article
human	IF	Nature Chromothripsis drives the evolution of gene amplification in cancer. Authors - Ofer Shoshani View Article
	WB	Nature Stella safeguards the oocyte methylome by preventing de novo methylation mediated by DNMT1. Authors - Yingfeng Li View Article
human	WB	Cell The Human RNA-Binding Proteome and Its Dynamics during Translational Arrest. Authors - Jakob Trendel View Article
mouse	WB	Cell Host Microbe Liberation of daidzein by gut microbial β-galactosidase suppresses acetaminophen-induced hepatotoxicity in mice Authors - Yunong Zeng View Article
human	WB	Cancer Discov Posttranslational regulation of the exon skipping machinery controls aberrant splicing in leukemia. Authors - Yalu Zhou View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH Mi (Verified Customer) (02-21-2023)	Works great in human adipocytes. Applications: Western Blot Primary Antibody Dilution: 1:2000 Cell Tissue Type: adipocytes
FH Ning (Verified Customer) (01-28-2023)	Excellent antibody to probe lamin B1. Applications: Western Blot Primary Antibody Dilution: 1:2000 Cell Tissue Type: mouse cortex lysate Lamin B1 Antibody Western Blot validation (1:2000 dilution) in mouse cortex lysate (Cat no:12987-1-AP)
FH Tsimafei (Verified Customer) (12-04-2022)	20µg of total protein were loaded. Incubation for 2h at room temperature. Excellent antibody Applications: Western Blot Primary Antibody Dilution: 1:10000 Cell Tissue Type: mESCs Lamin B1 Antibody Western Blot validation (1:10000 dilution) in mESCs (Cat no:12987-1-AP)
FH Alejandro (Verified Customer) (08-07-2022)	Works fine for flow cytometry Applications: Flow Cytometry Primary Antibody Dilution: 1/100 Cell Tissue Type: Mouse bone marrow derived macrophages
FH Iram (Verified Customer) (09-04-2020)	Very sharp bands for nuclear protein Applications: Western Blot Primary Antibody Dilution: 1:2000 for western Cell Tissue Type: Human colon cancer
FH Chun (Verified Customer) (12-05-2019)	Excellent antibody Applications: Western Blot, Primary Antibody Dilution: 1:5,000 Cell Tissue Type: HEK293 and HaCaT cells
FH Marco (Verified Customer) (09-02-2019)	good staining in conjunction with Alexa Fluor Plus 488 secondary Applications: Immunofluorescence, Primary Antibody Dilution: 1:100 Cell Tissue Type: human mesenchymal stem cells

Lamin B1 Polyclonal antibody

Lamin B1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

WB analysis using 12987-1-AP

WB analysis using 12987-1-AP

WB analysis of mouse lung using 12987-1-AP

WB analysis using 12987-1-AP

WB analysis of U-937 using 12987-1-AP

WB analysis of mouse spleen using 12987-1-AP

WB analysis of mouse pancreas using 12987-1-AP

WB analysis of SH-SY5Y using 12987-1-AP

WB analysis of K-562 using 12987-1-AP

WB analysis of Y79 using 12987-1-AP

WB analysis of mouse eye using 12987-1-AP

WB analysis of mouse kidney using 12987-1-AP

IP experiment of HeLa using 12987-1-AP

IHC staining of human liver cancer using 12987-1-AP

IHC staining of human kidney using 12987-1-AP

IHC staining of human kidney using 12987-1-AP

IHC staining of human colon cancer using 12987-1-AP

IF Staining of HepG2 using 12987-1-AP

IF Staining of HepG2 using 12987-1-AP

IF Staining of HepG2 using 12987-1-AP

IF Staining of NIH/3T3 using 12987-1-AP

IF Staining of human skin cancer using 12987-1-AP

FC experiment of HEK-293 using 12987-1-AP

"Lamin B1 Antibodies" Comparison

View side-by-side comparison of Lamin B1 antibodies from other vendors to find the one that best suits your research needs.

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases

Chromothripsis drives the evolution of gene amplification in cancer.

Stella safeguards the oocyte methylome by preventing de novo methylation mediated by DNMT1.

The Human RNA-Binding Proteome and Its Dynamics during Translational Arrest.

Liberation of daidzein by gut microbial β-galactosidase suppresses acetaminophen-induced hepatotoxicity in mice

Posttranslational regulation of the exon skipping machinery controls aberrant splicing in leukemia.

Reviews

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