Lamin B1 Polyclonal antibody
Lamin B1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat and More (5)
WB, IP, IHC, IF, FC, ChIP, ELISA
Cat no : 12987-1-AP
"Lamin B1 Antibodies" Comparison
View side-by-side comparison of Lamin B1 antibodies from other vendors to find the one that best suits your research needs.
|Positive WB detected in||HeLa cells, U-937 cells, mouse spleen tissue, K-562 cells, Y79 cells, mouse pancreas tissue, SH-SY5Y cells, mouse kidney tissue, mouse lung tissue, mouse eye tissue, HepG2 cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells, RAW 264.7 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human liver cancer tissue, human colon cancer tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, human skin cancer tissue, NIH/3T3 cells|
|Positive FC detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 2 publications below|
|WB||See 762 publications below|
|IHC||See 4 publications below|
|IF||See 42 publications below|
|FC||See 1 publications below|
|ChIP||See 2 publications below|
12987-1-AP targets Lamin B1 in WB, IP, IHC, IF, FC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, Bombyx mori, canine, chicken, hamster, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Lamin B1 fusion protein Ag3631|
|Full Name||lamin B1|
|Calculated molecular weight||66 kDa|
|Observed molecular weight||66-70 kDa|
|GenBank accession number||BC012295|
|Gene ID (NCBI)||4001|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Expression of uncleavable mutant lamin A or B caused significant delays in the onset of chromatin condensation and nuclear shrinkage during apoptosis (PMID:11953316). This protein is not suitable for samples where the nuclear envelope has been removed.
|Product Specific Protocols|
|WB protocol for Lamin B1 antibody 12987-1-AP||Download protocol|
|IHC protocol for Lamin B1 antibody 12987-1-AP||Download protocol|
|IF protocol for Lamin B1 antibody 12987-1-AP||Download protocol|
|IP protocol for Lamin B1 antibody 12987-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases
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Stella safeguards the oocyte methylome by preventing de novo methylation mediated by DNMT1.
The Human RNA-Binding Proteome and Its Dynamics during Translational Arrest.
Cell Host Microbe
Liberation of daidzein by gut microbial β-galactosidase suppresses acetaminophen-induced hepatotoxicity in mice
Posttranslational regulation of the exon skipping machinery controls aberrant splicing in leukemia.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Mi (Verified Customer) (02-21-2023)
Works great in human adipocytes.
Ning (Verified Customer) (01-28-2023)
Excellent antibody to probe lamin B1.
Tsimafei (Verified Customer) (12-04-2022)
20µg of total protein were loaded. Incubation for 2h at room temperature. Excellent antibody
Alejandro (Verified Customer) (08-07-2022)
Works fine for flow cytometry
Iram (Verified Customer) (09-04-2020)
Very sharp bands for nuclear protein
Chun (Verified Customer) (12-05-2019)
Marco (Verified Customer) (09-02-2019)
good staining in conjunction with Alexa Fluor Plus 488 secondary