MYL12A Antibody

MYL12A Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16287-1-AP

Synonyms

MLC 2B, MLCB, MRLC3, MYL12A, MYL2B, Myosin RLC, RLC



Tested Applications

Positive WB detected inNIH/3T3 cells, human skeletal muscle tissue, mouse bladder tissue, Jurkat cells, HEK-293 cells, HeLa cells, mouse heart tissue
Positive IHC detected inhuman heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

16287-1-AP targets MYL12A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYL12A fusion protein Ag9413
Full Name myosin, light chain 12A, regulatory, non-sarcomeric
Calculated molecular weight 171aa,20 kDa
Observed molecular weight 19-20 kDa
GenBank accession number BC016372
Gene symbol MYL12A
Gene ID (NCBI) 10627
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MYL12A antibody 16287-1-APDownload protocol
IHC protocol for MYL12A antibody 16287-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Biofizika

[Novel peptide inhibitors of the myosin light chain kinase suppress hyperpermeability of vascular endothelium].

Authors - Marchenko A V AV
pigWB

Biochem J

Kinase-related protein/telokin inhibits Ca2+-independent contraction in Triton-skinned guinea pig taenia coli.

Authors - Shcherbakova Olga V OV