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N-cadherin Polyclonal antibody

N-cadherin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 22018-1-AP

Synonyms

Cadherin 2, CD325, CDH2, CDHN, CDw325, N cadherin, NCAD, N-cadherin, Neural cadherin



Tested Applications

Positive WB detected inrat brain tissue, C2C12 cells, HEK-293 cells, mouse brain tissue, mouse heart tissue, C6 cells, rat heart tissue, HeLa cells
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse heart tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22018-1-AP is N-cadherin Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity cow, Gekko japonicus, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen N-cadherin fusion protein Ag16792
Full Name cadherin 2, type 1, N-cadherin (neuronal)
Calculated molecular weight 906 aa, 100 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC036470
Gene symbol CDH2
Gene ID (NCBI) 1000
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Neuronal cadherin (N-cadherin), also known as cadherin-2 (CDH2), is a calcium-binding protein that mediates cell-cell adhesions of neuronal and some non-neuronal cell types. 

What is the molecular weight of N-cadherin? Is N-cadherin post-translationally modified?

The molecular weight of mature N-cadherin is 127 kDa. N-cadherin is synthesized in a precursor form that undergoes proteolytic cleavage by furin at the Golgi apparatus. Additionally, it can be phosphorylated by casein kinase II and N-glycosylated, which affects its stability (PMID: 12604612 and 19846557). 

What is the subcellular localization of N-cadherin? What is the tissue expression pattern of N-cadherin?

N-cadherin is an integral membrane protein present at the plasma membrane, forming adherens junctions. It is widely expressed in the nervous system, where it flanks the active zone of synapses and is important for synapse formation and remodeling. It is also present in the lens, skeletal, and cardiac muscles (PMID: 3857614). In the muscle, N-cadherin plays a role in myoblast differentiation, while in the heart it is required for the formation of intercalated discs. Additionally, N-cadherin is present in blood vessels, promoting angiogenesis by forming adhesive complexes between endothelial cells and pericytes (PMID: 24521477). 

What is the role of N-cadherin during the epithelial-mesenchymal transition (EMT)?

EMT is a crucial process during gastrulation that leads to the formation of mesenchymal cells. It is marked by decreased expression of E-cadherin and upregulation of N-cadherin, which promotes cell migration (PMID: 23481201). Similarly, upregulation of N-cadherin is observed in many cancer cell types and is associated with increased invasiveness and metastasis.

Protocols

Product Specific Protocols
WB protocol for N-cadherin antibody 22018-1-APDownload protocol
IHC protocol for N-cadherin antibody 22018-1-APDownload protocol
IF protocol for N-cadherin antibody 22018-1-APDownload protocol
IP protocol for N-cadherin antibody 22018-1-APDownload protocol
FC protocol for N-cadherin antibody 22018-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Dose Response

Low-Dose Radiation Enhanced Inhibition of Breast Tumor Xenograft and Reduced Myocardial Injury Induced by Doxorubicin.

Authors - Xin Jiang
mouseWB,IHC

J Exp Clin Cancer Res

Tobacco smoke induced hepatic cancer stem cell-like properties through IL-33/p38 pathway.

Authors - Chunfeng Xie
humanWB

Mol Ther Nucleic Acids

The Attenuation of Trophoblast Invasion Caused by the Downregulation of EZH2 Is Involved in the Pathogenesis of Human Recurrent Miscarriage.

Authors - Shijian Lv
humanWB

Eur Rev Med Pharmacol Sci

Opa interacting protein 5 promotes metastasis of nasopharyngeal carcinoma cells by promoting EMT via modulation of JAK2/STAT3 signal.

Authors - Y-Q Zheng
humanWB

Urol Oncol

RPS7 promotes cell migration through targeting epithelial-mesenchymal transition in prostate cancer.

Authors - Yingwu Wen
humanWB

Hypertens Res

The potential role of pregnancy-associated plasma protein-A2 in angiogenesis and development of preeclampsia.

Authors - Xi Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Lianjie (Verified Customer) (07-26-2019)

Works very well.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Mouse embryonic heart
N-cadherin Antibody Immunofluorescence, validation (1:200 dilution) in Mouse embryonic heart (Cat no:22018-1-AP)
FH

Aurelie (Verified Customer) (06-13-2019)

Great antibody, no background, works also in human U2OS and primary fibroblasts with the same efficiency.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Mouse Cortex/human cell lines
N-cadherin Antibody Western Blot, validation (1/1000 dilution) in Mouse Cortex/human cell lines (Cat no:22018-1-AP)