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  • Featured Product
  • KD/KO Validated

NR3C2 Polyclonal antibody

NR3C2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 21854-1-AP

Synonyms

MCR, Mineralocorticoid receptor, MLR, MR, NR3C2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse kidney tissue using NR3C2 Polyclonal antibody (21854-1-AP)

    WB analysis of mouse kidney using 21854-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21854-1-AP (NR3C2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using NR3C2 Polyclonal antibody (21854-1-AP)

    WB analysis of HEK-293 using 21854-1-AP

    WB result of NR3C2 antibody (21854-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NR3C2 transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using NR3C2 Polyclonal antibody (21854-1-AP)

    WB analysis using 21854-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21854-1-AP (NR3C2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using NR3C2 Polyclonal antibody (21854-1-AP)

    IP experiment of HEK-293 using 21854-1-AP

    IP result of anti-NR3C2 (IP:21854-1-AP, 4ug; Detection:21854-1-AP 1:500) with HEK-293 cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using NR3C2 Polyclonal antibody (21854-1-AP)

    IHC staining of human kidney using 21854-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21854-1-AP (NR3C2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NR3C2 Polyclonal antibody (21854-1-AP)

    IHC staining of human kidney using 21854-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21854-1-AP (NR3C2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using NR3C2 Polyclonal antibody (21854-1-AP)

    IHC staining of human colon using 21854-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21854-1-AP (NR3C2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using NR3C2 Polyclonal antibody (21854-1-AP)

    IHC staining of human colon using 21854-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21854-1-AP (NR3C2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using NR3C2 Polyclonal antibody (21854-1-AP)

    IF Staining of HEK-293 using 21854-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using NR3C2 antibody (21854-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NR3C2 Polyclonal antibody (21854-1-AP)

    IF Staining of HepG2 using 21854-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NR3C2 antibody (21854-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inmouse kidney tissue, HEK-293 cells, HeLa cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21854-1-AP targets NR3C2 in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NR3C2 fusion protein Ag16410
Full Name nuclear receptor subfamily 3, group C, member 2
Calculated Molecular Weight 984 aa, 107 kDa
Observed Molecular Weight 94-110 kDa
GenBank Accession NumberBC111758
Gene Symbol NR3C2
Gene ID (NCBI) 4306
RRIDAB_10858785
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear receptor subfamily 3 group C member 2 (NR3C2), also known as Mineralocorticoid receptor (MCR or MR), is a member of the steroid/thyroid /retinoic nuclear hormone receptor superfamily that has been shown to activate gene transcription in response to aldosterone binding. Regulation of the mineralocorticoid receptors occurs through either receptor down-regulation (negative autoregulation) or hormone-mediated upregulation (positive autoregulation). MCR association with HSP 90 appears to be required for hormone binding to MCR and subsequent MCR activation.

Protocols

Product Specific Protocols
WB protocol for NR3C2 antibody 21854-1-APDownload protocol
IHC protocol for NR3C2 antibody 21854-1-APDownload protocol
IF protocol for NR3C2 antibody 21854-1-APDownload protocol
IP protocol for NR3C2 antibody 21854-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIHC

J Pineal Res

Melatonin prevents experimental central serous chorioretinopathy in rats.

Authors - Shanshan Yu
View Article
humanWB

EMBO Rep

High salt activates p97 to reduce host antiviral immunity by restricting Viperin induction.

Authors - Yukang Yuan
View Article
ratWB

Oncotarget

Impact of prenatal cold stress on placental physiology, inflammatory response, and apoptosis in rats.

Authors - Shuai Lian
View Article
ratIHC,IF

Int Immunopharmacol

UUO induces lung fibrosis with macrophage-myofibroblast transition in rats.

Authors - Fan Yang
View Article
ratWB

Behav Brain Res

Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring rats.

Authors - Shuai Lian
View Article
ratWB

Behav Brain Res

Prenatal cold stress: Effect on maternal hippocampus and offspring behavior in rats.

Authors - Shuai Lian
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Paloma (Verified Customer) (06-01-2022)

Good performance in ChIP

  • Applications: ChIP
  • Cell Tissue Type: Immortalized keratinocytes