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  • Featured Product
  • KD/KO Validated

NT5E,CD73 Monoclonal antibody

NT5E,CD73 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1G5G8

Cat no : 67789-1-Ig

Synonyms

5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), CD73, E5NT, Ecto 5 nucleotidase, eN, eNT, NT, NT5, NT5E, NT5E,CD73, NTE

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using NT5E,CD73 Monoclonal antibody (67789-1-Ig)

    WB analysis of HepG2 using 67789-1-Ig

    WB result of NT5E,CD73 antibody (67789-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NT5E,CD73 transfected HepG2 cells.

  • Western Blot (WB) analysis of various lysates using NT5E,CD73 Monoclonal antibody (67789-1-Ig)

    WB analysis using 67789-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67789-1-Ig (NT5E,CD73 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using NT5E,CD73 Monoclonal antibody (67789-1-Ig)

    IHC staining of human tonsillitis using 67789-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67789-1-Ig (NT5E,CD73 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using NT5E,CD73 Monoclonal antibody (67789-1-Ig)

    IHC staining of human tonsillitis using 67789-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67789-1-Ig (NT5E,CD73 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using NT5E,CD73 Monoclonal antibody (67789-1-Ig)

    IHC staining of rat kidney using 67789-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67789-1-Ig (NT5E,CD73 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using NT5E,CD73 Monoclonal antibody (67789-1-Ig)

    IHC staining of rat kidney using 67789-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67789-1-Ig (NT5E,CD73 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using NT5E,CD73 Monoclonal antibody (67789-1-Ig)

    IF Staining of human tonsillitis using 67789-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using NT5E,CD73 antibody (67789-1-Ig, Clone: 1G5G8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of rat liver tissue using NT5E,CD73 Monoclonal antibody (67789-1-Ig)

    IF Staining of rat liver using 67789-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using NT5E,CD73 antibody (67789-1-Ig, Clone: 1G5G8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of rat liver tissue using NT5E,CD73 Monoclonal antibody (67789-1-Ig)

    IF Staining of rat liver using 67789-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using NT5E,CD73 antibody (67789-1-Ig, Clone: 1G5G8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, A549 cells, HT-29 cells, U-87 MG cells
Positive IHC detected inhuman tonsillitis tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue, rat liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67789-1-Ig targets NT5E,CD73 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NT5E,CD73 fusion protein Ag30616
Full Name 5'-nucleotidase, ecto (CD73)
Calculated Molecular Weight 29 kDa, 63 kDa
Observed Molecular Weight60-70 kDa
GenBank Accession NumberBC015940
Gene Symbol NT5E
Gene ID (NCBI) 4907
RRIDAB_2918553
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).

Protocols

Product Specific Protocols
WB protocol for NT5E,CD73 antibody 67789-1-IgDownload protocol
IHC protocol for NT5E,CD73 antibody 67789-1-IgDownload protocol
IF protocol for NT5E,CD73 antibody 67789-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols