OLIG2 Polyclonal antibody

OLIG2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13999-1-AP

Synonyms

BHLHB1, bHLHe19, OLIG2, OLIGO2, PRKCBP2, RACK17



Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inrat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)IF : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

13999-1-AP targets OLIG2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OLIG2 fusion protein Ag5089
Full Name oligodendrocyte lineage transcription factor 2
Calculated molecular weight 32 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC047511
Gene symbol OLIG2
Gene ID (NCBI) 10215
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

What is the specificity of Olig2?

Oligodendrocyte transcription factor 2 (OLIG2) is expressed by cells found in the central nervous system (CNS) called oligodendrocyte precursor cells (OPCs), which form the myelin sheaths wrapping the axons of neurons in the brain and spinal cord. OPCs differentiate into oligodendrocytes that form the myelin, providing metabolic support and saltatory conduction. OLIG2 is expressed broadly throughout their development to OPCs. This protein can be used to identify many cells of the oligodendrocyte lineage. It is found mostly in the nucleoplasm but also in the cytoplasm.

 

What is the function of OLIG2?

The basic helix-loop-helix structure of OLIG2 allows it to function as a transcription factor, determining cell fate in the development of neural tissue, where it is located at the pMN domain in the embryonic spinal cord. Expression of OLIG2 causes neural precursors to develop into oligodendrocytes or into motor neurons and expression is then maintained postnatally. OLIG2 co-operates with other factors to cause this differentiation from precursors, although overexpression alone can cause differentiation to the oligodendrocyte lineage.1 The continued expression of OLIG2 in OPCs indicates an ongoing role in the maintenance of their stemness.

 

What is the involvement of OLIG2 in disease?

The expression of OLIG2 in glioblastoma, the most common type of malignant brain tumor in adults, is well characterized, where the pathological function is an extension of the normal function. Stem-like cells that propagate the tumor growth have been shown to be OLIG2-expressing, and are one of the key transcription factors involved in the re-programming of differentiated cells of the tumor to stem-like cells.2 OLIG2 has also been associated with demyelinating diseases like multiple sclerosis (MS), as OPCs have been shown to be involved in the process of remyelination.3

 

1.     Liu, Z. et al. Induction of oligodendrocyte differentiation by Olig2 and Sox10: Evidence for reciprocal interactions and dosage-dependent mechanisms. Dev. Biol. 302, 683–693 (2007).

2.     Wegener, A. et al. Gain of Olig2 function in oligodendrocyte progenitors promotes remyelination. Brain 138, 120–35 (2015).

3.     Ettle, B., Schlachetzki, J. C. M. & Winkler, J. Oligodendroglia and Myelin in Neurodegenerative Diseases: More Than Just Bystanders? Mol. Neurobiol. 53, 3046–3062 (2016). 

Protocols

Product Specific Protocols
WB protocol for OLIG2 antibody 13999-1-APDownload protocol
IHC protocol for OLIG2 antibody 13999-1-APDownload protocol
IF protocol for OLIG2 antibody 13999-1-APDownload protocol
IP protocol for OLIG2 antibody 13999-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

J Cell Biochem

Chondroitin sulfate proteoglycan represses neural stem/progenitor cells migration via PTPσ/α-actinin4 signaling pathway.

Authors - Jun Zhong
humanIF

Brain Res

G Protein-Coupled Estrogen Receptor 1 Negatively Regulates the Proliferation of Mouse-Derived Neural Stem/Progenitor Cells via Extracellular Signal-regulated Kinase Pathway.

Authors - Jun Zhong
ratIHC

Front Neurosci

Myelin Damage in Diffuse Axonal Injury.

Authors - Jiao Mu
ratIHC

Heliyon

Restorative effect of modified dioscorea pills on the structure of hippocampal neurovascular unit in an animal model of chronic cerebral hypoperfusion.

Authors - H B Li
mouseIF

J Comp Neurol

Distribution of endogenous normal tau in the mouse brain.

Authors - Atsuko Kubo
ratIF

Biotechniques

Antibody incubation at 37°C improves fluorescent immunolabeling in free-floating thick tissue sections.

Authors - Xia Xiao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

An (Verified Customer) (09-17-2020)

Tried a free aliquot of this antibody to perform IHC on retinal cryosections of zebrafish. Did not work, although it was to be expected as the sequence homology was not great.

  • Applications: Immunohistochemistry
FH

Alexander (Verified Customer) (08-05-2019)

This is a great product that has a clear nuclear stain at a 1:200 dilution and 1 hour primary incubation at room temp and 1 hour secondary incubation at room temp.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: brain organoid/neural progenitor cells