OLIG2 Polyclonal antibody

OLIG2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13999-1-AP

Synonyms

BHLHB1, bHLHe19, OLIG2, OLIGO2, PRKCBP2, RACK17



Tested Applications

Positive WB detected inmouse brain tissue, rat brain
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inrat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13999-1-AP targets OLIG2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OLIG2 fusion protein Ag5089
Full Name oligodendrocyte lineage transcription factor 2
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 32-36 kDa
GenBank Accession NumberBC047511
Gene Symbol OLIG2
Gene ID (NCBI) 10215
RRIDAB_2157541
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

What is the specificity of Olig2?

Oligodendrocyte transcription factor 2 (OLIG2) is expressed by cells found in the central nervous system (CNS) called oligodendrocyte precursor cells (OPCs), which form the myelin sheaths wrapping the axons of neurons in the brain and spinal cord. OPCs differentiate into oligodendrocytes that form the myelin, providing metabolic support and saltatory conduction. OLIG2 is expressed broadly throughout their development to OPCs. This protein can be used to identify many cells of the oligodendrocyte lineage. It is found mostly in the nucleoplasm but also in the cytoplasm.

 

What is the function of OLIG2?

The basic helix-loop-helix structure of OLIG2 allows it to function as a transcription factor, determining cell fate in the development of neural tissue, where it is located at the pMN domain in the embryonic spinal cord. Expression of OLIG2 causes neural precursors to develop into oligodendrocytes or into motor neurons and expression is then maintained postnatally. OLIG2 co-operates with other factors to cause this differentiation from precursors, although overexpression alone can cause differentiation to the oligodendrocyte lineage.1 The continued expression of OLIG2 in OPCs indicates an ongoing role in the maintenance of their stemness.

 

What is the involvement of OLIG2 in disease?

The expression of OLIG2 in glioblastoma, the most common type of malignant brain tumor in adults, is well characterized, where the pathological function is an extension of the normal function. Stem-like cells that propagate the tumor growth have been shown to be OLIG2-expressing, and are one of the key transcription factors involved in the re-programming of differentiated cells of the tumor to stem-like cells.2 OLIG2 has also been associated with demyelinating diseases like multiple sclerosis (MS), as OPCs have been shown to be involved in the process of remyelination.3

 

1.     Liu, Z. et al. Induction of oligodendrocyte differentiation by Olig2 and Sox10: Evidence for reciprocal interactions and dosage-dependent mechanisms. Dev. Biol. 302, 683-693 (2007).

2.     Wegener, A. et al. Gain of Olig2 function in oligodendrocyte progenitors promotes remyelination. Brain 138, 120-35 (2015).

3.     Ettle, B., Schlachetzki, J. C. M. & Winkler, J. Oligodendroglia and Myelin in Neurodegenerative Diseases: More Than Just Bystanders? Mol. Neurobiol. 53, 3046-3062 (2016). 

Protocols

Product Specific Protocols
WB protocol for OLIG2 antibody 13999-1-APDownload protocol
IHC protocol for OLIG2 antibody 13999-1-APDownload protocol
IF protocol for OLIG2 antibody 13999-1-APDownload protocol
IP protocol for OLIG2 antibody 13999-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nature

Suppression of proteolipid protein rescues Pelizaeus-Merzbacher disease.

Authors - Matthew S Elitt
mouseIF

Nat Biomed Eng

Variants of the adeno-associated virus serotype 9 with enhanced penetration of the blood-brain barrier in rodents and primates

Authors - Yizheng Yao
mouseIHC

Cell Stem Cell

Non-canonical Targets of HIF1a Impair Oligodendrocyte Progenitor Cell Function.

Authors - Kevin C Allan
mouseWB

J Pineal Res

Melatonin pretreatment alleviates the long-term synaptic toxicity and dysmyelination induced by neonatal Sevoflurane exposure via MT1 receptor-mediated Wnt signaling modulation.

Authors - Lirong Liang
human

Neuron

Suppression of premature transcription termination leads to reduced mRNA isoform diversity and neurodegeneration.

Authors - Geneva R LaForce
mouseIF

Neuron

Macrophages Expressing GALC Improve Peripheral Krabbe Disease by a Mechanism Independent of Cross-Correction.

Authors - Nadav I Weinstock

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Reyes (Verified Customer) (03-01-2024)

Olig2 (in red) marked the nuclei of tiny cells (oligodendrocytes) around the neurons (in green)

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Human Brain Cortex
OLIG2 Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:13999-1-AP)
FH

Clarisse (Verified Customer) (08-29-2022)

This antibody works very well (clear nuclear signal).

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Human cerebral organoids
OLIG2 Antibody Immunofluorescence validation (1:500 dilution) in Human cerebral organoids (Cat no:13999-1-AP)
FH

An (Verified Customer) (09-17-2020)

Tried a free aliquot of this antibody to perform IHC on retinal cryosections of zebrafish. Did not work, although it was to be expected as the sequence homology was not great.

  • Applications: Immunohistochemistry
FH

Alexander (Verified Customer) (08-05-2019)

This is a great product that has a clear nuclear stain at a 1:200 dilution and 1 hour primary incubation at room temp and 1 hour secondary incubation at room temp.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: brain organoid/neural progenitor cells