|Positive WB detected in||HEK-293 cells, HeLa cells, HepG2 cells|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human gliomas tissue, mouse ovary tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
60097-1-Ig targets PCNA in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, pig, rat samples.
|Tested Reactivity||human, mouse, pig, rat|
|Cited Reactivity||human, mouse, pig, rabbit, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||PCNA fusion protein Ag7416|
|Full Name||proliferating cell nuclear antigen|
|Calculated molecular weight||29 kDa/31 kDa|
|Observed molecular weight||36 kDa|
|GenBank accession number||BC000491|
|Gene ID (NCBI)||5111|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa （PMID: 1358458）.
|Product Specific Protocols|
|WB protocol for PCNA antibody 60097-1-Ig||Download protocol|
|IHC protocol for PCNA antibody 60097-1-Ig||Download protocol|
|IF protocol for PCNA antibody 60097-1-Ig||Download protocol|
|IP protocol for PCNA antibody 60097-1-Ig||Download protocol|
|FC protocol for PCNA antibody 60097-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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