Biotin-conjugated PCNA Monoclonal antibody

PCNA Monoclonal Antibody for IHC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig, rat

Applications

IHC

Conjugate

Biotin Fluorescent Dye

CloneNo.

10D10E11

Cat no : Biotin-60097

Synonyms

Cyclin, PCNA



Tested Applications

Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

Biotin-60097 targets PCNA in IHC applications and shows reactivity with human, mouse, pig, rat samples.

Tested Reactivity human, mouse, pig, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PCNA fusion protein Ag7416
Full Name proliferating cell nuclear antigen
Calculated molecular weight 29 kDa/31 kDa
GenBank accession numberBC000491
Gene symbol PCNA
Gene ID (NCBI) 5111
Conjugate Biotin Fluorescent Dye
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa (PMID: 1358458).

Protocols

Product Specific Protocols
IHC protocol for Biotin PCNA antibody Biotin-60097Download protocol
Standard Protocols
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