Biotin-conjugated PCNA Monoclonal antibody
PCNA Monoclonal Antibody for IHC
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, pig, rat
Applications
WB, IHC
Conjugate
Biotin
CloneNo.
10D10E11
Cat no : Biotin-60097
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
Product Information
Biotin-60097 targets PCNA in WB, IHC applications and shows reactivity with human, mouse, pig, rat samples.
Tested Reactivity | human, mouse, pig, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | PCNA fusion protein Ag7416 |
Full Name | proliferating cell nuclear antigen |
Calculated Molecular Weight | 29 kDa/31 kDa |
Observed Molecular Weight | 36-38 kDa |
GenBank Accession Number | BC000491 |
Gene Symbol | PCNA |
Gene ID (NCBI) | 5111 |
RRID | AB_2883063 |
Conjugate | Biotin |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa (PMID: 1358458).
Protocols
Product Specific Protocols | |
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IHC protocol for Biotin PCNA antibody Biotin-60097 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Death Dis PTEN alleviates maladaptive repair of renal tubular epithelial cells by restoring CHMP2A-mediated phagosome closure. |