Tested Applications
| Positive WB detected in | RAW 264.7 cells, human lymph node tissue, rat spleen tissue, mouse thymus tissue, Jurkat cells, MOLT-4 cells, THP-1 cells, CTLL-2 cells, pig thymus tissue |
| Positive IHC detected in | human tonsillitis tissue, human appendicitis tissue, human lung cancer tissue, human lymphoma tissue, human tonsillitis tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue, human lymphoma tissue, mouse spleen tissue, mouse thymus tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66220-1-Ig targets PD-1/CD279 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12470 Product name: Recombinant human PD-1/CD279 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-288 aa of BC074740 Sequence: MQIPQAPWPVVWAVLQLGWRPGWFLDSPDRPWNPPTFSPALLVVTEGDNATFTCSFSNTSESFVLNWYRMSPSNQTDKLAAFPEDRSQPGQDCRFRVTQLPNGRDFHMSVVRARRNDSGTYLCGAISLAPKAQIKESLRAELRVTERRAEVPTAHPSPSPRPAGQFQTLVVGVVGGLLGSLVLLVWVLAVICSRAARGTIGARRTGQPLKEDPSAVPVFSVDYGELDFQWREKTPEPPVPCVPEQTEYATIVFPSGMGTSSPARRGSADGPRSAQPLRPEDGHCSWPL Predict reactive species |
| Full Name | programmed cell death 1 |
| Calculated Molecular Weight | 288 aa, 32 kDa |
| Observed Molecular Weight | 32 kDa, 47-55 kDa |
| GenBank Accession Number | BC074740 |
| Gene Symbol | PD-1 |
| Gene ID (NCBI) | 5133 |
| RRID | AB_2881611 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q15116 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). The calculated molecular weight of PD-1 is 32 kDa. It has been reported that PD-1 is heavily glycosylated and migrates with an apparent molecular mass of 47-55 kDa on SDS-PAGE (PMID: 8671665; 17640856; 17003438).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PD-1/CD279 antibody 66220-1-Ig | Download protocol |
| IHC protocol for PD-1/CD279 antibody 66220-1-Ig | Download protocol |
| WB protocol for PD-1/CD279 antibody 66220-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
PD-1/CD279 Monoclonal antibody (4H4D1), catalog number 66220-1-Ig, has accumulated 107 citations across more than 60 journals, including Nature Communications, Cell, Cell Metabolism, Nature Genetics, Journal of Clinical Investigation, Advanced Science, Science Advances, and Frontiers in Immunology. Associated research fields include cancer immunotherapy, immune checkpoint regulation, tumor microenvironment immunosuppression, neuroinflammation, metabolic reprogramming, autoimmune diseases, and pain management.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Chemo-photothermal synergy ignites antitumor immunity via ferroptosis. | ||
Cell Metab Hepatic acetyl-CoA metabolism modulates neuroinflammation and depression susceptibility via acetate | ||
Cell Metab Macrophage PD-1 regulates energy expenditure and metabolic dysfunction under immune checkpoint blockade.
| ||
Cancer Commun (Lond) Metabolic landscape of head and neck squamous cell carcinoma informs a novel kynurenine/Siglec-15 axis in immune escape | ||
Nat Genet Single-cell multi-omic and spatial profiling of esophageal squamous cell carcinoma reveals the immunosuppressive role of GPR116+ pericytes in cancer metastasis |
Reviews
What customers say
All three reviewers gave 5-star ratings. Two used the antibody for Western Blot at dilutions of 1:1000 and 1:5000, reporting clear bands in human cell lines (HL-60, NK-92) and mouse thymus. One reviewer used it for IHC on paraffin-embedded hamster lung tissue at 1:100 with Tris-EDTA (pH 9) antigen retrieval, confirming successful staining. Overall, users consistently report strong, specific results across multiple species and applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Wiesława (Verified Customer) (12-08-2022) | works very well
 |
Macarena Lucia (Verified Customer) (10-17-2022) | clear band
|
Mona (Verified Customer) (10-17-2021) | Staining of paraffin-embedded lung tissue from hamster. Antigen retrieval was performed with Tris-EDTA (pH 9)
|
