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  • KD/KO Validated

PDCD6 Antibody

PDCD6 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12303-1-AP

Synonyms

ALG 2, ALG2, PDCD6, PEF1B, programmed cell death 6



Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, mouse liver tissue, rat liver tissue, NIH/3T3 cells, K-562 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12303-1-AP targets PDCD6 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDCD6 fusion protein Ag2949
Full Name programmed cell death 6
Calculated molecular weight 191aa,22 kDa
Observed molecular weight 17-22 kDa
GenBank accession number BC012384
Gene symbol PDCD6
Gene ID (NCBI) 10016
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PDCD6 antibody 12303-1-APDownload protocol
IHC protocol for PDCD6 antibody 12303-1-APDownload protocol
IP protocol for PDCD6 antibody 12303-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncol Lett

Luteinizing hormone inhibits cisplatin-induced apoptosis in human epithelial ovarian cancer cells.

Authors - Lingfang Xia
humanWB

AIDS

IL-27 posttranslationally regulates Y-box binding protein-1 to inhibit HIV-1 replication in human CD4+ T cells.

Authors - Deepak Poudyal
humanWB

Endocr Relat Cancer

FSH inhibits ovarian cancer cell apoptosis by up-regulating survivin and down-regulating PDCD6 and DR5.

Authors - Huang Yan Y
humanWB,IF

Cell

Regulation of the CUL3 Ubiquitin Ligase by a Calcium-Dependent Co-adaptor.

Authors - Colleen A McGourty
  • KD Validated
humanIF

Science

Triggered recruitment of ESCRT machinery promotes endolysosomal repair.

Authors - Michael L Skowyra