|Positive WB detected in||HEK-293 cells, Jurkat cells, HeLa cells, HepG2 cells, K-562 cells, NIH/3T3 cells, mouse liver tissue, rat liver tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human lung cancer tissue, human stomach tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12303-1-AP targets PDCD6 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PDCD6 fusion protein Ag2949|
|Full Name||programmed cell death 6|
|Calculated molecular weight||191 aa, 22 kDa|
|Observed molecular weight||22 kDa|
|GenBank accession number||BC012384|
|Gene ID (NCBI)||10016|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
J Exp Clin Cancer Res
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Luteinizing hormone inhibits cisplatin-induced apoptosis in human epithelial ovarian cancer cells.
Genetic screening of ANXA11 revealed novel mutations linked to amyotrophic lateral sclerosis.
IL-27 posttranslationally regulates Y-box binding protein-1 to inhibit HIV-1 replication in human CD4+ T cells.
Endocr Relat Cancer
FSH inhibits ovarian cancer cell apoptosis by up-regulating survivin and down-regulating PDCD6 and DR5.
Regulation of the CUL3 Ubiquitin Ligase by a Calcium-Dependent Co-adaptor.