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  • KD/KO Validated

PDCD6 Polyclonal antibody

PDCD6 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12303-1-AP

Synonyms

ALG 2, ALG2, PDCD6, PEF1B, programmed cell death 6



Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, HeLa cells, HepG2 cells, K-562 cells, NIH/3T3 cells, mouse liver tissue, rat liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12303-1-AP targets PDCD6 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDCD6 fusion protein Ag2949
Full Name programmed cell death 6
Calculated molecular weight 191 aa, 22 kDa
Observed molecular weight22 kDa
GenBank accession numberBC012384
Gene symbol PDCD6
Gene ID (NCBI) 10016
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PDCD6 antibody 12303-1-APDownload protocol
IHC protocol for PDCD6 antibody 12303-1-APDownload protocol
IP protocol for PDCD6 antibody 12303-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Exp Clin Cancer Res

PDCD6 cooperates with C-Raf to facilitate colorectal cancer progression via Raf/MEK/ERK activation.

Authors - Xiaojuan Wang
  • KD Validated
humanWB

Oncol Lett

Luteinizing hormone inhibits cisplatin-induced apoptosis in human epithelial ovarian cancer cells.

Authors - Lingfang Xia
humanWB

AIDS

IL-27 posttranslationally regulates Y-box binding protein-1 to inhibit HIV-1 replication in human CD4+ T cells.

Authors - Deepak Poudyal
humanWB

Endocr Relat Cancer

FSH inhibits ovarian cancer cell apoptosis by up-regulating survivin and down-regulating PDCD6 and DR5.

Authors - Huang Yan Y
humanWB,IF

Cell

Regulation of the CUL3 Ubiquitin Ligase by a Calcium-Dependent Co-adaptor.

Authors - Colleen A McGourty
  • KD Validated
humanIF

Science

Triggered recruitment of ESCRT machinery promotes endolysosomal repair.

Authors - Michael L Skowyra