Phospholipase C Beta 1 Polyclonal antibody

Phospholipase C Beta 1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 26551-1-AP

Synonyms

KIAA0581, Phospholipase C beta 1, Phospholipase C I, PI PLC, PLC 154, PLC beta 1, PLC I, PLC154, PLCB1



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman gliomas tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26551-1-AP is Phospholipase C Beta 1 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Phospholipase C Beta 1 fusion protein Ag24750
Full Name phospholipase C, beta 1 (phosphoinositide-specific)
Calculated molecular weight 1216 aa, 139 kDa
Observed molecular weight 139 kDa
GenBank accession numberBC069420
Gene symbol PLCB1
Gene ID (NCBI) 23236
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLCB1 catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals. PLCB1 is activated by two G-protein alpha subunits, alpha-q and alpha-11.

Protocols

Product Specific Protocols
WB protocol for Phospholipase C Beta 1 antibody 26551-1-APDownload protocol
IHC protocol for Phospholipase C Beta 1 antibody 26551-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols