Tested Applications
| Positive WB detected in | SH-SY5Y cells, rat brain tissue, U-251 cells, mouse brain tissue, rat tissue |
| Positive IHC detected in | human gliomas tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 7 publications below |
| IHC | See 2 publications below |
| RIP | See 1 publications below |
Product Information
26551-1-AP targets Phospholipase C Beta 1 in WB, IHC, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat, sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24750 Product name: Recombinant human PLCB1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1162-1216 aa of BC069420 Sequence: LEFVQEAMKGKISEDSNHGSAPLSLSSDPGKVNHKTPSSEELGGDIPGKEFDTPL Predict reactive species |
| Full Name | phospholipase C, beta 1 (phosphoinositide-specific) |
| Calculated Molecular Weight | 1216 aa, 139 kDa |
| Observed Molecular Weight | 139 kDa |
| GenBank Accession Number | BC069420 |
| Gene Symbol | PLCB1 |
| Gene ID (NCBI) | 23236 |
| RRID | AB_2861360 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NQ66 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PLCB1 catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals. PLCB1 is activated by two G-protein alpha subunits, alpha-q and alpha-11.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Phospholipase C Beta 1 antibody 26551-1-AP | Download protocol |
| WB protocol for Phospholipase C Beta 1 antibody 26551-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biochem Genet PLCB1 Enhances Cell Migration and Invasion in Gastric Cancer Via Regulating Actin Cytoskeletal Remodeling and Epithelial-Mesenchymal Transition
| ||
J Ethnopharmacol Combining metabolomics and network pharmacology to clarify the mechanism of Angelica sinensis-Sophora flavescens pills in treating atopic dermatitis through the sweet-bitter harmonization theory | ||
J Ethnopharmacol Dan-Shen-Yin against ethanol-induced chronic gastric mucosal injury in rats by inhibiting apoptosis via IP3R-controlled calcium release | ||
Int J Mol Sci Elucidating the Role of circTIAM1 in Guangling Large-Tailed Sheep Adipocyte Proliferation and Differentiation via the miR-485-3p/PLCB1 Pathway | ||
Front Endocrinol (Lausanne) Estrogen receptor subtype mediated anti-inflammation and vasorelaxation via genomic and nongenomic actions in septic mice | ||
Thorac Cancer LncRNA AC100826.1 regulated PLCB1 to promote progression in non-small cell lung cancer |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Matteo (Verified Customer) (12-12-2025) | Very clear and specific bands. It was used at lower suggested concnetration and the bands very strong and clear after 60 seconds of exposure.
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