PRPH2 Polyclonal antibody

PRPH2 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 18109-1-AP

Synonyms

AOFMD, AVMD, Peripherin 2, Peripherin2, PRPH, PRPH2, rd2, RDS, RP7, Tetraspanin 22, Tspan 22, TSPAN22



Tested Applications

Positive WB detected inmouse eye tissue
Positive IF detected inmouse Retina tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 18109-1-AP is PRPH2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRPH2 fusion protein Ag12555
Full Name peripherin 2 (retinal degeneration, slow)
Calculated molecular weight 346 aa, 39 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC074720
Gene symbol PRPH2
Gene ID (NCBI) 5961
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Peripherin-2 (PRPH2), also known as retinal degeneration slow protein (RDS), is a photoreceptor-specific tetraspanin protein implicated in outer segment disk morphogenesis. It may function as an adhesion molecule involved in stabilization and compaction of outer segment disks or in the maintenance of the curvature of the rim. Mutations in peripherin-2 are responsible for various retinal degenerative diseases including autosomal dominant retinitis pigmentosa (ADRP).

Protocols

Product Specific Protocols
WB protocol for PRPH2 antibody 18109-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

J Neurosci

The Late Endosomal Pathway Regulates the Ciliary Targeting of Tetraspanin Protein Peripherin 2.

Authors - Wataru Otsu
ratWB,IP

Genes Cells

Multimeric conformation of type III intermediate filaments but not the filamentous conformation exhibits high affinity to lipid bilayers.

Authors - Beomju Hwang
mouseWB, IHC

PLoS One

Differential requirement of NPHP1 for compartmentalized protein localization during photoreceptor outer segment development and maintenance.

Authors - Poppy Datta
mouseWB,IF

J Biol Chem

The myosin-tail homology domain of centrosomal protein 290 is essential for protein confinement between the inner and outer segments in photoreceptors.

Authors - Poppy Datta
mouseIF

Sci Rep

Evaluation of micro Electroretinograms Recorded with Multiple Electrode Array to Assess Focal Retinal Function.

Authors - Momo Fujii
mouseIHC

Hum Mol Genet

BBS mutations modify phenotypic expression of CEP290-related ciliopathies.

Authors - Zhang Yan Y