RPL18A Polyclonal antibody

RPL18A Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14653-1-AP

Synonyms

60S ribosomal protein L18a, ribosomal protein L18a, RPL18A



Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, human brain tissue, mouse lung tissue, PC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

14653-1-AP targets RPL18A in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL18A fusion protein Ag6251
Full Name ribosomal protein L18a
Calculated molecular weight 21 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC066319
Gene symbol RPL18A
Gene ID (NCBI) 6142
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RPL18A antibody 14653-1-APDownload protocol
IHC protocol for RPL18A antibody 14653-1-APDownload protocol
IF protocol for RPL18A antibody 14653-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Genetics

Quantitative changes in gimap3 and gimap5 expression modify mitochondrial DNA segregation in mice.

Authors - Riikka Jokinen
humanWB

Sci Rep

Down-regulation of HSP60 Suppresses the Proliferation of Glioblastoma Cells via the ROS/AMPK/mTOR Pathway.

Authors - Haiping Tang