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S100A4 Antibody

S100A4 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2G11B4

Cat no : 66489-1-Ig

Synonyms

18A2, 42A, Calvasculin, CAPL, Fibroblast-specific protein 1, FSP1, FSP-1, Metastasin, MTS1, P9KA, PEL98, Protein Mts1, Protein S100 A4, S100A4



Tested Applications

Positive WB detected inHeLa cells, A549 cells, A375 cells, PC-3 cells, NIH/3T3 cells
Positive IHC detected inhuman lung cancer tissue, human tonsil tissue, human stomach cancer tissue, human tonsillitis tissue, mouse lung tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66489-1-Ig targets S100A4 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen S100A4 fusion protein ag9019
Full Name S100 calcium binding protein A4
Calculated molecular weight 101 aa, 12 kDa
Observed molecular weight 12 kDa
GenBank accession numberBC016300
Gene symbol S100A4
Gene ID (NCBI) 6275
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

S100A4 is a member of the S100 family of calcium-binding proteins. The S100 family members have been involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A4 is known to localize to and function in the nucleus, cytoplasm of cells, and the extracellular space. S100A4 has also been shown to be associated with tumor growth, motility, invasion, metastasis, angiogenesis, apoptosis, and chemoresistance. It is a fibroblast-specific protein associated with mesenchymal cell morphology and motility, is expressed during epithelial-mesenchymal transformations (EMT) in vivo (PMID: 9362334). It is a specific prognostic marker for renal survival in patients with IgAN (PMID: 16105038). It is also an improved marker for lung fibroblasts that could be useful for investigating the pathogenesis of pulmonary fibrosis(PMID: 15618458). Overexpression of S100A4 is correlated with a worse prognosis inpatients with various types of cancer.

Protocols

Product Specific Protocols
WB protocol for S100A4 antibody 66489-1-IgDownload protocol
IHC protocol for S100A4 antibody 66489-1-IgDownload protocol
IF protocol for S100A4 antibody 66489-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols