|Positive WB detected in||HeLa cells, Jurkat cells, NIH/3T3 cells, PC-3 cells, Ramos cells, A431 cells, A549 cells, MCF-7 cells, Jurkat cells|
|Positive IP detected in||U2OS cells, Jurkat cells|
|Positive IHC detected in||human colon cancer tissue, human breast cancer tissue, human stomach cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
10508-1-AP targets SURVIVIN in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||hamster, human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SURVIVIN fusion protein Ag0788|
|Full Name||baculoviral IAP repeat-containing 5|
|Calculated molecular weight||16 kDa|
|Observed molecular weight||16-18 kDa|
|GenBank accession number||BC008718|
|Gene ID (NCBI)||332|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Survivin, also called BIRC5, is a unique member of the inhibitor of apoptosis (IAP) protein family. Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy, but is completely absent in terminally differentiated cells. The differential expression of survivin in cancer versus normal tissues makes it a useful tool in cancer diagnosis and a promising therapeutic target. Survivin expression is also highly regulated by the cell cycle and is only expressed in the G2-M phase. It is known that survivin localizes to the mitotic spindle by interaction with tubulin during mitosis and may play a contributing role in regulating mitosis. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. It also is a direct target gene of the Wnt pathway and is upregulated by beta-catenin.
Clin Sci (Lond)
Myricetin Suppresses the Propagation of Hepatocellular Carcinoma via Down-Regulating Expression of YAP.
Synergistic effects of 7-O-geranylquercetin and siRNAs on the treatment of human breast cancer.
A Noncoding Regulatory RNAs Network Driven by Circ-CDYL Acts Specifically in the Early Stages Hepatocellular Carcinoma.
J Oral Pathol Med
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Susmita (Verified Customer) (08-21-2020)
Giving good result and crisp band in Western Blot.
Iram (Verified Customer) (08-14-2020)
We got very sharp and clean band of survivin with this antibody.