Tested Applications
| Positive WB detected in | HeLa cells, PC-3 cells, Jurkat cells, NIH/3T3 cells, A549 cells, MCF-7 cells, A431 cells, Ramos cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human colon cancer tissue, human breast cancer tissue, human stomach cancer tissue, human urothelial carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue |
| Positive IF/ICC detected in | MCF-7 cells, HeLa cells |
| Positive FC (Intra) detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10508-1-AP targets SURVIVIN in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, canine, zebrafish, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0788 Product name: Recombinant human SURVIVIN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-142 aa of BC008718 Sequence: MGAPTLPPAWQPFLKDHRISTFKNWPFLEGCACTPERMAEAGFIHCPTENEPDLAQCFFCFKELEGWEPDDDPIEEHKKHSSGCAFLSVKKQFEELTLGEFLKLDRERAKNKIAKETNNKKKEFEETAKKVRRAIEQLAAMD Predict reactive species |
| Full Name | baculoviral IAP repeat-containing 5 |
| Calculated Molecular Weight | 16 kDa |
| Observed Molecular Weight | 16-18 kDa |
| GenBank Accession Number | BC008718 |
| Gene Symbol | Survivin |
| Gene ID (NCBI) | 332 |
| RRID | AB_2064048 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15392 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Survivin, also called BIRC5, is a unique member of the inhibitor of apoptosis (IAP) protein family. Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy, but is completely absent in terminally differentiated cells. The differential expression of survivin in cancer versus normal tissues makes it a useful tool in cancer diagnosis and a promising therapeutic target. Survivin expression is also highly regulated by the cell cycle and is only expressed in the G2-M phase. It is known that survivin localizes to the mitotic spindle by interaction with tubulin during mitosis and may play a contributing role in regulating mitosis. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. It also is a direct target gene of the Wnt pathway and is upregulated by beta-catenin.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for SURVIVIN antibody 10508-1-AP | Download protocol |
| IF protocol for SURVIVIN antibody 10508-1-AP | Download protocol |
| IHC protocol for SURVIVIN antibody 10508-1-AP | Download protocol |
| IP protocol for SURVIVIN antibody 10508-1-AP | Download protocol |
| WB protocol for SURVIVIN antibody 10508-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-Survivin (BIRC5) antibody, catalog number 10508-1-AP, has accumulated 190 citations. Publications appear in high-impact journals including Nature Communications, Cancer Research, Autophagy, Hepatology, Circulation Research, ACS Nano, Theranostics, Advanced Science, Cell Death & Disease, Cancer Letters, Biomaterials, and Clinical & Translational Medicine. Research fields predominantly span oncology (hepatocellular, lung, breast, colorectal, prostate, ovarian cancers), autophagy, pulmonary hypertension, rheumatoid arthritis, nanomedicine-based drug delivery, and apoptosis/cell death signaling.
| Species | Application | Title |
|---|---|---|
Cancer Res USP10 Promotes Proliferation of Hepatocellular Carcinoma by Deubiquitinating and Stabilizing YAP/TAZ. | ||
Autophagy Elaiophylin, a novel autophagy inhibitor, exerts antitumor activity as a single agent in ovarian cancer cells. | ||
Autophagy ATF6 supports lysosomal function in tumor cells to enable ER stress-activated macroautophagy and CMA: impact on mutant TP53 expression | ||
Nat Commun Gemcitabine plus selinexor in selective advanced sarcomas: a phase I of the Spanish group for research on sarcoma study. | ||
Circ Res Iron Homeostasis and Pulmonary Hypertension: Iron Deficiency Leads to Pulmonary Vascular Remodeling in the Rat. | ||
Hepatology A Noncoding Regulatory RNAs Network Driven by Circ-CDYL Acts Specifically in the Early Stages Hepatocellular Carcinoma. |
Reviews
What customers say
All six reviews are five-star ratings. Users consistently praise the Survivin antibody for producing clear, sharp, and crisp bands in Western Blot, with several also reporting good performance in immunohistochemistry. It was tested at dilutions ranging from 1:400 to 1:2000 across various samples including colon cancer cells, CRC cells, and pancreatic cell lines. Reviewers describe it as reliable, high-quality, and well-suited for their projects, with no negative feedback reported.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Mounika (Verified Customer) (03-24-2026) | Helpful, great performance
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Iram (Verified Customer) (03-23-2026) | Very good survivin antibody
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Sai Sindhura (Verified Customer) (03-23-2026) | Survivin antibody works very good for my project
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Iram (Verified Customer) (09-18-2025) | Bands are very clear band. Very good quality.
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Susmita (Verified Customer) (08-21-2020) | Giving good result and crisp band in Western Blot.
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Iram (Verified Customer) (08-14-2020) | We got very sharp and clean band of survivin with this antibody.
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