Tested Applications
| Positive WB detected in | HepG2 cells, HEK-293 cells, HeLa cells, MCF-7 cells, mouse heart tissue, mouse brain tissue, rat brain tissue, NIH/3T3 cells, C6 cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 25 publications below |
| IHC | See 4 publications below |
| IF | See 8 publications below |
| IP | See 1 publications below |
Product Information
18900-1-AP targets ZO-2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13534 Product name: Recombinant human TJP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-289 aa of BC027592 Sequence: MPVRGDRGFPPRRELSGWLRAPGMEELIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKVAAIVVKRPRKVQVAALQASPPLDQDDRAFEVMDEFDGRSFRSGYSERSRLNSHGGRSRSWEDSPERGRPHERARSRERDLSRDRSRGRSLERGLDQDHARTRDRSRGRSLERGLDHDFGPSRDRDRDRSRGRSIDQDYERAYHRAYDPDYERAYSPEYRRGARHDARSRGPRSRSREH Predict reactive species |
| Full Name | tight junction protein 2 (zona occludens 2) |
| Calculated Molecular Weight | 134 kDa |
| Observed Molecular Weight | 150 kDa |
| GenBank Accession Number | BC027592 |
| Gene Symbol | TJP2 |
| Gene ID (NCBI) | 9414 |
| RRID | AB_2203584 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UDY2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Zona occludens protein 2 (ZO-2), also known as tight junction protein 2 (TJP2), is a membrane-associated guanylate kinase acting at intercellular contacts of epithelial and endothelial cells but also in their nuclei.
What is the molecular weight of ZO-2? Are there any isoforms of ZO-2? Is ZO-2 post-translationally modified?
The molecular weight of ZO-2 is 160 kDa. There are two known isoforms of ZO-2: full-length ZO-2A and ZO-2C lacking the first 23 amino acids (PMID: 10360833). ZO-2 can be phosphorylated and O-glycosylated, which has an impact on its localization (PMID: 23804652).
What is the subcellular localization of ZO-2?
ZO-2 is present at the cytoplasmic face at cell-cell contact sites: tight junctions, adherens junctions, and gap junctions (PMID: 20224657). ZO-2 contains nuclear localization and nuclear export signals and can actively shuttle between the cytoplasm and nucleus (PMID: 11855865). In the nucleus it can affect the activity of various transcription factors. ZO-2 nuclear localization is most prominent in sparse 2D cell cultures and also in cells subjected to mechanical or chemical stress.
What is the tissue expression pattern of ZO-2?
ZO-2 is expressed in various tissues in epithelial and endothelial cells. ZO-2 isoforms have tissue specificity, with ZO-2A being abundant in the heart and brain, while ZO-2C is found in the kidney, pancreas, heart, and placenta (PMID: 11018256).
What proteins can be used as markers of tight junctions?
Zona occludens proteins are often used as tight junction markers. Apart from the antibody against ZO-2, one can use antibodies against ZO-1 (https://www.ptglab.com/products/ZO1-Antibody-21773-1-AP.htm), occludin (https://www.ptglab.com/products/OCLN-Antibody-13409-1-AP.htm), and claudins (e.g., claudin 1: https://www.ptglab.com/products/CLDN1-Antibody-13050-1-AP.htm).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ZO-2 antibody 18900-1-AP | Download protocol |
| IHC protocol for ZO-2 antibody 18900-1-AP | Download protocol |
| WB protocol for ZO-2 antibody 18900-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO Mol Med Deficiency in intestinal epithelial O-GlcNAcylation predisposes to gut inflammation. | ||
Mater Today Bio Endoscopic nasal delivery of engineered endothelial progenitor cell-derived exosomes improves angiogenesis and neurological deficits in rats with intracerebral hemorrhage | ||
Cell Rep p120 RasGAP and ZO-2 are essential for Hippo signaling and tumor-suppressor function mediated by p190A RhoGAP
| ||
Biomater Sci Improved epithelial cell-cell adhesion using molecular mobility of supramolecular surfaces. | ||
Free Radic Biol Med Prenylated xanthones from mangosteen (Garcinia mangostana) activate the AhR and Nrf2 pathways and protect intestinal barrier integrity in HT-29 cells. | ||
J Agric Food Chem Gut Microbiota from Short-Chain Chlorinated Paraffin-Exposed Mice Promotes Astrocyte Activation by Disrupting the Intestinal Tight Junction via Zonulin Upregulation |
