ZO-2 Polyclonal antibody

ZO-2 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18900-1-AP

Synonyms

Tight junction protein 2, Tight junction protein ZO 2, TJP2, X104, ZO 2, ZO2, ZO-2, Zona occludens protein 2, Zonula occludens protein 2



Tested Applications

Positive WB detected inMCF-7 cells, HEK-293 cells, HeLa cells, mouse heart tissue, mouse brain tissue, rat brain tissue, NIH/3T3 cells, C6 cells
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

18900-1-AP targets ZO-2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZO-2 fusion protein Ag13534
Full Name tight junction protein 2 (zona occludens 2)
Calculated molecular weight 134 kDa
Observed molecular weight 150 kDa
GenBank accession numberBC027592
Gene symbol TJP2
Gene ID (NCBI) 9414
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Zona occludens protein 2 (ZO-2), also known as tight junction protein 2 (TJP2), is a membrane-associated guanylate kinase acting at intercellular contacts of epithelial and endothelial cells but also in their nuclei.

What is the molecular weight of ZO-2? Are there any isoforms of ZO-2? Is ZO-2 post-translationally modified?

The molecular weight of ZO-2 is 160 kDa. There are two known isoforms of ZO-2: full-length ZO-2A and ZO-2C lacking the first 23 amino acids (PMID: 10360833). ZO-2 can be phosphorylated and O-glycosylated, which has an impact on its localization (PMID: 23804652).

What is the subcellular localization of ZO-2?

ZO-2 is present at the cytoplasmic face at cell-cell contact sites: tight junctions, adherens junctions, and gap junctions (PMID: 20224657). ZO-2 contains nuclear localization and nuclear export signals and can actively shuttle between the cytoplasm and nucleus (PMID: 11855865). In the nucleus it can affect the activity of various transcription factors. ZO-2 nuclear localization is most prominent in sparse 2D cell cultures and also in cells subjected to mechanical or chemical stress.

What is the tissue expression pattern of ZO-2?

ZO-2 is expressed in various tissues in epithelial and endothelial cells. ZO-2 isoforms have tissue specificity, with ZO-2A being abundant in the heart and brain, while ZO-2C is found in the kidney, pancreas, heart, and placenta (PMID: 11018256).

What proteins can be used as markers of tight junctions?

Zona occludens proteins are often used as tight junction markers. Apart from the antibody against ZO-2, one can use antibodies against ZO-1 (https://www.ptglab.com/products/ZO1-Antibody-21773-1-AP.htm), occludin (https://www.ptglab.com/products/OCLN-Antibody-13409-1-AP.htm), and claudins (e.g., claudin 1: https://www.ptglab.com/products/CLDN1-Antibody-13050-1-AP.htm).


Protocols

Product Specific Protocols
WB protocol for ZO-2 antibody 18900-1-APDownload protocol
IF protocol for ZO-2 antibody 18900-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

Acta Pharmacol Sin

Activation of SIRT1 ameliorates LPS-induced lung injury in mice via decreasing endothelial tight junction permeability.

Authors - Cuiping Fu
WB

J Cereb Blood Flow Metab

NLRP3 deficiency ameliorates neurovascular damage in experimental ischemic stroke.

Authors - Fan Yang
mouseIF

EMBO Mol Med

Deficiency in intestinal epithelial O-GlcNAcylation predisposes to gut inflammation.

Authors - Ming Zhao