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VDAC1/Porin Polyclonal antibody

VDAC1/Porin Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 55259-1-AP

Synonyms

hVDAC1, Plasmalemmal porin, Porin, PORIN 31 HL, Porin 31HL, Porin 31HM, VDAC, VDAC 1, VDAC1



Tested Applications

Positive WB detected inRAW 264.7 cells, A431 cells, HEK-293 cells, HeLa cells, HepG2 cells, human skeletal muscle tissue, mouse heart tissue, mouse liver tissue, rat liver tissue, ROS1728 cells, mouse brain tissue, rat brain tissue, NIH/3T3 cells, rat heart tissue
Positive IP detected inA431 cells
Positive IHC detected inhuman heart tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

55259-1-AP targets VDAC1/Porin in WB, IP, IHC, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity hamsters, human, mouse, rat, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name voltage-dependent anion channel 1
Calculated molecular weight 31 kDa
Observed molecular weight 31 kDa
GenBank accession numberNM_003374
Gene symbol VDAC1
Gene ID (NCBI) 7416
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

VDAC1, also named as VDAC, porin 31HM, porin 31HL and plasmalemmal porin, belongs to the eukaryotic mitochondrial porin family. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, to form a channel through the mitochondrial outer membrane and also the plasma membrane. Unlike other membrane transport proteins, porins are large enough to allow passive diffusion. Studies have shown that VDAC1 is subject to both phosphorylation and acetylation (PMID: 23233904). The apparent molecular weight of VDAC1 is 30-37 kDa (PMID: 14573604; 23754752; 25681439). Hypoxic conditions were found to trigger cleavage of the VDAC1 C-terminal to yield a 26-kDa truncated but active form (PMID: 22389449; 23233904). This antibody is specific to VDAC1.

Protocols

Product Specific Protocols
WB protocol for VDAC1/Porin antibody 55259-1-APDownload protocol
IHC protocol for VDAC1/Porin antibody 55259-1-APDownload protocol
IP protocol for VDAC1/Porin antibody 55259-1-APDownload protocol
FC protocol for VDAC1/Porin antibody 55259-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Eur J Pharmacol

A novel ent-kaurane diterpenoid analog, DN3, selectively kills human gastric cancer cells via acting directly on mitochondria.

Authors - Yong-Cheng Ma
hamstersWB,IHC

Int J Mol Sci

PMID: 30845718

Authors - Syed Zahid Ali Shah
mouseWB

EBioMedicine

DsbA-L ameliorates high glucose induced tubular damage through maintaining MAM integrity.

Authors - Ming Yang
humanWB

Cells

Perilipin 5 Protects against Cellular Oxidative Stress by Enhancing Mitochondrial Function in HepG2 Cells.

Authors - Yanjie Tan

Mol Genet Metab Rep

Neonatal mitochondrial hepatoencephalopathy caused by novel GFM1 mutations.

Authors - Kirstine Ravn
humanWB,CoIP

J Cell Mol Med

Novel mitochondrion-targeting copper(II) complex induces HK2 malfunction and inhibits glycolysis via Drp1-mediating mitophagy in HCC.

Authors - Mengmeng Li