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ZG16 Monoclonal antibody

ZG16 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig, rat and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A7B9

Cat no : 67389-1-Ig

Synonyms

FLJ43571, FLJ92276, hZG16, JCLN, JCLN1, Secretory lectin ZG16, ZG16, ZG16A, Zymogen granule protein 16

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse colon tissue using ZG16 Monoclonal antibody (67389-1-Ig)

    WB analysis of mouse colon using 67389-1-Ig

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 67389-1-Ig (ZG16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human colon tissue using ZG16 Monoclonal antibody (67389-1-Ig)

    WB analysis of human colon using 67389-1-Ig

    human colon tissue were subjected to SDS PAGE followed by western blot with 67389-1-Ig (ZG16 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig colon tissue using ZG16 Monoclonal antibody (67389-1-Ig)

    WB analysis of pig colon using 67389-1-Ig

    pig colon tissue were subjected to SDS PAGE followed by western blot with 67389-1-Ig (ZG16 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using ZG16 Monoclonal antibody (67389-1-Ig)

    IHC staining of mouse pancreas using 67389-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67389-1-Ig (ZG16 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using ZG16 Monoclonal antibody (67389-1-Ig)

    IHC staining of mouse pancreas using 67389-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67389-1-Ig (ZG16 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using ZG16 Monoclonal antibody (67389-1-Ig)

    IF Staining of mouse small intestine using 67389-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ZG16 antibody (67389-1-Ig, Clone: 1A7B9 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), ASCT2 antibody (20350-1-AP, green), CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-80008, Clone: 5H7, Magenta).

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using ZG16 Monoclonal antibody (67389-1-Ig)

    IF Staining of mouse small intestine using 67389-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ZG16 antibody (67389-1-Ig, Clone: 1A7B9 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), ASCT2 antibody (20350-1-AP, green), CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-80008, Clone: 5H7, Magenta).

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using ZG16 Monoclonal antibody (67389-1-Ig)

    IF Staining of mouse small intestine using 67389-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ZG16 antibody (67389-1-Ig, Clone: 1A7B9 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), ASCT2 antibody (20350-1-AP, green), CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-80008, Clone: 5H7, Magenta).

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using ZG16 Monoclonal antibody (67389-1-Ig)

    IF Staining of mouse small intestine using 67389-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ZG16 antibody (67389-1-Ig, Clone: 1A7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using ZG16 Monoclonal antibody (67389-1-Ig)

    IF Staining of mouse small intestine using 67389-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ZG16 antibody (67389-1-Ig, Clone: 1A7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inmouse colon tissue, human colon tissue, pig colon tissue
Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse small intestine tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

67389-1-Ig targets ZG16 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, pig, rat samples.

Tested Reactivity Human, mouse, pig, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ZG16 fusion protein Ag11332
Full Name zymogen granule protein 16 homolog (rat)
Calculated Molecular Weight 167 aa, 18 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC029149
Gene Symbol ZG16
Gene ID (NCBI) 653808
RRIDAB_2882633
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zymogen granule protein 16 (ZG16) has a Jacalin-like lectin domain, which is mainly expressed by mucus-secreting cells, including goblet cells in the intestine. It's reported that ZG16 expression was significantly decreased in colorectal cancer compared to normal tissue. ZG16 gene expression and copy number variations (CNV) were associated with multiple molecular and clinicopathological features of CRC including MSI, MLH1 silencing and so on. It's found that ZG16 is negatively correlated with lymphatic invasive and distant metastasis. Besides, overexpression of ZG16 blocks PD-L1 expression in colorectal cancer, and promotes NK cells survival and proliferation. Very importantly, ZG16 suppresses colorectal tumor growth via the immune system. (PMID: 33360840, 21893569)

Protocols

Product Specific Protocols
WB protocol for ZG16 antibody 67389-1-IgDownload protocol
IHC protocol for ZG16 antibody 67389-1-IgDownload protocol
IF protocol for ZG16 antibody 67389-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Elife

Proteomic characteristics reveal the signatures and the risks of T1 colorectal cancer metastasis to lymph nodes

Authors - Aojia Zhuang
View Article