|Positive WB detected in||NIH/3T3 cells, HEK-293 cells, C6 cells, A375 cells, mouse ovary tissue, HUVEC cells, SKOV-3 cells|
|Positive IP detected in||A375 cells|
|Positive IF detected in||HepG2 cells, HeLa cells|
|Positive FC detected in||HEK-293T cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10298-1-AP targets Lamin A/C in WB, IP, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||duck, human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Lamin A/C fusion protein Ag0408|
|Full Name||lamin A/C|
|Calculated molecular weight||65 kDa|
|Observed molecular weight||65 kDa, 70 kDa|
|GenBank accession number||BC003162|
|Gene ID (NCBI)||4000|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Lamin A/C is also named as LMNA, or LMN1. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. The lack of lamin A/C can be as a novel marker for undifferentiated embryonic stem cells and lamin A/C expression is as an early indicator of differentiation (PMID: 16179429). Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. This protein has 4 isoforms produced by alternative splicing with the molecular weight of 74 kDa, 65 kDa, 70 kDa and 64 kDa. This antibody can recognize 4 isoforms of Lamin A/C.
|Product Specific Protocols|
|WB protocol for Lamin A/C antibody 10298-1-AP||Download protocol|
|IF protocol for Lamin A/C antibody 10298-1-AP||Download protocol|
|IP protocol for Lamin A/C antibody 10298-1-AP||Download protocol|
|FC protocol for Lamin A/C antibody 10298-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Cell Death Dis
Angiomotin-p130 inhibits β-catenin stability by competing with Axin for binding to tankyrase in breast cancer.
Biochim Biophys Acta Mol Basis Dis
Deficiency of heat shock protein A12A promotes browning of white adipose tissues in mice.
Inhibition of Influenza A Virus Replication by TRIM14 via Its Multifaceted Protein-Protein Interaction With NP.
Mol Med Rep
miR‑494‑3p regulates lipopolysaccharide‑induced inflammatory responses in RAW264.7 cells by targeting PTEN.
Biochim Biophys Acta Gene Regul Mech
HSF1 phosphorylation by cyclosporin A confers hyperthermia sensitivity through suppression of HSP expression.
JNK activation-mediated nuclear SIRT1 protein suppression contributes to silica nanoparticle-induced pulmonary damage via p53 acetylation and cytoplasmic localisation.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Declan (Verified Customer) (11-29-2018)