Antibody Validation Policy
Proteintech is committed to rigorous and transparent antibody validation. Read our policy to learn how we put this commitment into practice.
KEY POINTS
Original Manufacturer
Proteintech is the only broad-catalog reagent provider that manufactures and validates its primary antibodies entirely in-house, providing complete oversight of the antibody life cycle - from antigen design and antibody production through to validation and quality control. As the original manufacturer, Proteintech generates and retains the primary validation data for every lot of its product portfolio, ensuring full traceability of experimental data and enabling continuous quality assessment throughout each product’s lifetime.
Target-Specific Validation
Proteintech employs a target-specific validation strategy in which each antibody is validated across multiple applications using physiologically relevant models selected according to the characteristics of the target protein. Appropriate controls and genetic approaches, such as knockout (KO) or knockdown (KD) validation, are used to confirm target specificity in Western blot and immunofluorescence assays. Additional orthogonal and application-specific validation methods, such as ELISA, flow cytometry, immunoprecipitation, and biolayer interferometry, are further incorporated to verify fit-for-purpose antibodies. This multi-application validation approach is performed for every lot, ensuring consistent lot-to-lot performance for each target.
Multi-application validation approach is performed for every lot, ensuring consistent lot-to-lot performance for each target.
Complementing this approach, epitope mapping of single clones is performed to characterize antibody-target interactions. This represents an emerging standard in antibody validation as it confirms target specificity at the amino-acid level, improving predictions of cross-reactivity, and providing a mechanistic understanding of antibody binding that supports long-term reproducibility.
Overall, antibody performance is evaluated at the molecular level and under experimental conditions that are fit-for-purpose and reflective of the underlying biology of the target.
Data Integrity and Transparency
Proteintech is committed to preserving the integrity of its published validation data. Images are presented without digital manipulation that could alter the interpretation of experimental results. To improve clarity and readability, single-lane Western blots are displayed only when they are taken from a membrane that has been probed for multiple targets, allowing the relevant validation result to be highlighted without altering the underlying experimental data. In all other cases, published validation data remains unedited.
To provide greater transparency in immunohistochemistry (IHC) validation, Proteintech provides interactive whole slide images that allow researchers to evaluate an antibody the same way they would examine a stained tissue section under a microscope. Rather than relying on a single carefully selected representative image, this transparent, open-access approach enables users to inspect the entire specimen and independently assess staining quality, increasing confidence that the data accurately reflects antibody performance.
Data Traceability
Proteintech validation data are available in the product page gallery and can be filtered by application. AbleAI further streamlines access to validation data, protocols, and supporting citations. Every validation experiment is fully traceable to the Proteintech scientist who performed it through internal quality management systems and data records.
For further information, researchers may request the original raw data files associated with validation experiments by contacting our technical support teams. These files retain their original timestamps and metadata, providing a complete audit trail from data acquisition to the published results.
By maintaining complete control over antibody manufacturing and characterization, Proteintech provides researchers with dependable reagents supported by comprehensive validation data and full traceability.