Tested Applications
| Positive WB detected in | HGC-27 cells, HUVEC cells, LNCaP cells, MKN-45 cells, K-562 cells, human peripheral blood platelets, rat heart tissue, mouse heart tissue, rabbit heart tissue, HeLa cells |
| Positive IHC detected in | human ovary cancer tissue, human placenta tissue, human heart tissue, rat heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | hTERT-RPE1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66305-2-Ig targets Vinculin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.
| Tested Reactivity | human, mouse, rat, rabbit |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24946 Product name: Recombinant human Vinculin protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 777-1066 aa of BC039174 Sequence: PKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ Predict reactive species |
| Full Name | vinculin |
| Calculated Molecular Weight | 1133 aa, 124 kDa |
| Observed Molecular Weight | 124 kDa |
| GenBank Accession Number | BC039174 |
| Gene Symbol | Vinculin |
| Gene ID (NCBI) | 7414 |
| RRID | AB_3670375 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P18206 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein that is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Vinculin antibody 66305-2-Ig | Download protocol |
| IHC protocol for Vinculin antibody 66305-2-Ig | Download protocol |
| WB protocol for Vinculin antibody 66305-2-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Vinculin Monoclonal antibody (3A11F8), catalog number 66305-2-Ig, has 17 citations. These appear in journals including Cell Stem Cell, Science Advances, EMBO Journal, Advanced Science, Cell Death & Disease, Journal of Medicinal Chemistry, and others. Research fields span breast cancer metastasis, osteosarcoma, melanoma immunotherapy, cerebral ischemia, hepatic fibrosis, subarachnoid hemorrhage, Huntington's disease, autism, autoinflammation, osteoarthritis, and transcription factor biology. All cited applications are Western blot.
| Species | Application | Title |
|---|---|---|
Cell Stem Cell Paired patient-derived organoids reveal transcription factor-driven epigenetic remodeling in breast cancer metastasis. | ||
Adv Sci (Weinh) RFWD3 Reprograms Nucleotide Metabolism Through PHGDH to Induce Chemoresistance In Osteosarcoma | ||
Sci Adv Engineered anchoring aptamers induce relocalization and functional inactivation of transcription factors. | ||
Cell Death Dis Nucleoporin TPR integrates MAPK signaling with mitogen-induced transcriptional programs. | ||
Phytomedicine Unraveling ancient wisdom: The molecular mechanism of Gujin Luyan Xuming decoction in combating cerebral ischemia-reperfusion injury | ||
Mater Today Bio A purpurin-based metalloimmunostimulant amplifies ROS and modulates STING/TNF-α axis to potentiate melanoma immunotherapy. |
Reviews
What customers say
All three reviews are 5-star ratings from Western Blot applications at a 1:1000 dilution on human fibroblasts. Users consistently report a clear, well-defined band at the expected molecular weight. The antibody is described as a reliable housekeeping control, with one reviewer explicitly recommending the product. Overall, the feedback highlights strong specificity, consistent performance, and ease of use for routine Western blotting.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Matthieu (Verified Customer) (09-24-2025) | Band is very clear at the expected size
|
Manon (Verified Customer) (09-24-2025) | Good housekeeping antibody
|
Emilie (Verified Customer) (07-23-2025) | Used for Western blot on human fibroblasts and gives a clear, well-defined band. I recommend this product.
|
