CRHR1 Recombinant monoclonal antibody

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• CRHR1 Recombinant monoclonal antibody (87114-1-RR)

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  WB analysis using 87114-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87114-1-RR (CRHR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

CRHR1 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87114-1-RR

Clone No.251134H2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Corticotropin-releasing factor receptor 1, Corticotropin-releasing hormone receptor 1, CRF R, CRF R 1, CRF R1

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Tested Applications

• Positive WB detected in: HeLa cells, SH-SY5Y cells, Y79 cells, HT-29 cells, rat brain tissue, mouse brain tissue, mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87114-1-RR targets CRHR1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: corticotropin releasing hormone receptor 1
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: NM_004382
• Gene Symbol: CRHR1
• Gene ID (NCBI): 1394
• RRID: AB_3745349
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P34998
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Corticotropin-releasing hormone (CRH, also known as Corticotropin-releasing factor, CRF) is a 41 amino acid peptide hormone and a key regulator of the stress response. CRH and the related urocortin peptides (UCN, UCN2 and UCN3) mediate their actions through two mammalian receptor subtypes, CRHR1 and CRHR2 (PMID: 23863939). CRHR1 (also known as CRFR1) exhibits high affinity towards CRH and UCN but low affinity towards UCN2 and no affinity towards UCN3 (PMID: 22684850). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Widely distributed throughout the brain, CRHR1 critically controls behavioral adaptation to stress and is causally linked to emotional disorders (PMID: 21885734). Alternative splicing results in multiple transcript variants encoding distinct isoforms of CRHR1. This antibody raised against a synthesized peptide corresponding to amino acid residues 148-165 of isoform CRF-R1 can only recognize isoform CRF-R1.