Anticorps Polyclonal de lapin anti-APAF1

• Phare
• Validé par KD/KO

APAF1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

43

N° de cat : 21710-1-AP

Synonymes

APAF 1, APAF1, CED4, KIAA0413

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Galerie de données de validation

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  WB analysis of HEK-293 using 21710-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21710-1-AP (APAF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 21710-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 21710-1-AP (APAF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 21710-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21710-1-AP (APAF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 21710-1-AP

  IP Result of anti-APAF1 (IP:21710-1-AP, 4ug; Detection:21710-1-AP 1:500) with HEK-293 cells lysate 2000ug.

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  IHC staining of human heart using 21710-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 21710-1-AP (APAF1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 21710-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 21710-1-AP (APAF1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human heart using 21710-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21710-1-AP (APAF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 21710-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21710-1-AP (APAF1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A549 using 21710-1-AP

  Immunofluorescent analysis of A549 cells using 21710-1-AP (APAF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A549, cellules COLO 320
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 42 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

21710-1-AP cible APAF1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: APAF1 Protéine recombinante Ag16336
• Nom complet: apoptotic peptidase activating factor 1
• Masse moléculaire calculée: 1248 aa, 142 kDa
• Poids moléculaire observé: 130-142 kDa
• Numéro d’acquisition GenBank: BC136532
• Symbole du gène: APAF1
• Identification du gène (NCBI): 317
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

APAF1, also named as Apoptotic protease-activating factor 1 or KIAA0413, is a 1248 amino acid protein, which contains one CARD domain, contains one NB-ARC domain and Contains fifteen WD repeats. APAF1 localizes in the cytoplasm and is widely expressed in many tissues. Highest levels of expression is in adult spleen, peripheral blood leukocytes, in fetal brain, kidney and lung. Oligomeric APAF1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.

Publications

Species	Application	Title
mouse	WB	Theranostics; Pro-Inflammatory CXCR3 Impairs Mitochondrial Function in Experimental Non-Alcoholic Steatohepatitis.; Authors - Jinghua Du
human	WB	Oxid Med Cell Longev; Apoptotic Protease Activating Factor-1 Inhibitor Mitigates Myocardial Ischemia Injury via Disturbing Procaspase-9 Recruitment by Apaf-1.; Authors - Ying Wang
human	WB,IHC,IF	Mol Ther Nucleic Acids; LNC473 Regulating APAF1 IRES-Dependent Translation via Competitive Sponging miR574 and miR15b: Implications in Colorectal Cancer.; Authors - Huizhe Wu
rat	WB	Cell Biol Toxicol; Inhibition of ER stress attenuates kidney injury and apoptosis induced by 3-MCPD via regulating mitochondrial fission/fusion and Ca2+ homeostasis.; Authors - Yujie Zhong
human	WB	Front Cell Dev Biol; Antagonizing CDK8 Sensitizes Colorectal Cancer to Radiation Through Potentiating the Transcription of e2f1 Target Gene apaf1.; Authors - Bin Chen
human	WB	Cells; FUT2 Facilitates Autophagy and Suppresses Apoptosis via p53 and JNK Signaling in Lung Adenocarcinoma Cells; Authors - Yuqi Zhang

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