Anticorps Monoclonal anti-P53

• Phare
• Validé par KD/KO

P53 Monoclonal Antibody for ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

23

CloneNo.

2D2F10

N° de cat : 60283-1-Ig

Synonymes

Antigen NY CO 13, Cellular tumor antigen p53, LFS1, Phosphoprotein p53, TRP53, tumor protein p53, Tumor suppressor p53

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Galerie de données de validation

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  WB analysis of COLO 320 using 60283-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 60283-1-Ig (P53 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 60283-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60283-1-Ig (P53 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 60283-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60283-1-Ig (P53 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 60283-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 60283-1-Ig (P53 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 60283-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60283-1-Ig (P53 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 60283-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60283-1-Ig (P53 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 60283-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60283-1-Ig (P53 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 60283-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60283-1-Ig (P53 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

Dilution recommandée

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 22 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

60283-1-Ig cible P53 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: P53 Protéine recombinante Ag0698
• Nom complet: tumor protein p53
• Masse moléculaire calculée: 44 kDa
• Numéro d’acquisition GenBank: BC003596
• Symbole du gène: TP53
• Identification du gène (NCBI): 7157
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TP53, also named as P53 and NY-CO-13, belongs to the p53 family. It has 9 isoforms. In SDS-Page, the MW is about 53kd. TP53 acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. The antibody reacts specifically with 53 kDa protein.

Publications

Species	Application	Title
human	WB	Mol Ther Nucleic Acids; miR-552 Regulates Liver Tumor-Initiating Cell Expansion and Sorafenib Resistance.; Authors - Tao Han
human	WB	Oncogenesis; MeCP2 facilitates breast cancer growth via promoting ubiquitination-mediated P53 degradation by inhibiting RPL5/RPL11 transcription.; Authors - DongDong Tong
human	WB,IHC	Cancer Lett; TAZ regulates cell proliferation and sensitivity to vitamin D3 in intrahepatic cholangiocarcinoma.; Authors - Heng Xiao ; KD Validated
human,mouse	WB	Am J Cancer Res; Asparaginyl endopeptidase (AEP) regulates myocardial apoptosis in response to radiation exposure via alterations in NRF2 activation.; Authors - Lu Cao
human	WB	Am J Cancer Res; Loss of BRMS2 induces cell growth inhibition and translation capacity reduction in colorectal cancer cells.; Authors - Yaofu Liu
human	WB	Biochem Pharmacol; Ketoplatin in triple-negative breast cancer cells MDA-MB-231: High efficacy and low toxicity, and positive impact on inflammatory microenvironment.; Authors - Zhong-Ying Ma

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