human BCL2 Polyclonal antibody

• Featured Product
• KD/KO Validated

human BCL2 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (9)

Applications

WB, IP, IHC, IF, FC, CoIP, chIP, ELISA

Conjugate

Unconjugated

Publications

2075

Reviews (5)

Cat no : 12789-1-AP

Synonyms

Apoptosis regulator Bcl 2, B cell CLL/lymphoma 2, BCL2, BCL-2, Oncogene B cell leukemia 2

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Validation Data Gallery

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  WB analysis of A431 using 12789-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12789-1-AP (BCL2 antibody) at dilution of 1:9000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat cells using 12789-1-AP

  WB result of BCL2 (12789-1-AP, 1:1000) with si-Control and si-BCL2 transfected Jurkat cells.

• 

  WB analysis using 12789-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12789-1-AP (human BCL2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12789-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12789-1-AP (BCL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12789-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12789-1-AP (BCL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12789-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12789-1-AP (BCL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-251 using 12789-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 12789-1-AP (BCL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HL-60 using 12789-1-AP

  IP result of anti-BCL2 (IP:12789-1-AP, 4ug; Detection:12789-1-AP 1:1000) with HL-60 cells lysate 4000 ug.

• 

  IP experiment of HepG2 using 12789-1-AP

  IP Result of anti-BCL2 (IP:12789-1-AP, 3ug; Detection:12789-1-AP 1:500) with HepG2 cells lysate 4500ug.

• 

  IP experiment of MCF-7 using 12789-1-AP

  IP Result of anti-BCL2 (IP:12789-1-AP, 3ug; Detection:12789-1-AP 1:1000) with MCF-7 cells lysate 2500ug.

• 

  IHC staining of human tonsillitis using 12789-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12789-1-AP (BCL2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 12789-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12789-1-AP (BCL2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 12789-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12789-1-AP (BCL2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 12789-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12789-1-AP (BCL2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of Jurkat using 12789-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human BCL2 (12789-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of human peripheral blood lymphocytes using 12789-1-AP

  1X10^6 human peripheral blood lymphocytes were surface stained with 0.2 ug Anti-Human BCL2 (12789-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 4% PFA.

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Tested Applications

• Positive WB detected in: A431 cells, HepG2 cells, Jurkat cells, HEK-293 cells, HL-60 cells, U-251 cells, U-937 cells, MCF-7 cells, U2OS cells
• Positive IP detected in: HL-60 cells, HepG2 cells, MCF-7 cells
• Positive IHC detected in: human tonsillitis tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: Jurkat cells, human peripheral blood lymphocytes

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:300

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 2003 publications below
• IHC: See 100 publications below
• IF: See 34 publications below
• IP: See 2 publications below
• FC: See 1 publications below
• CoIP: See 6 publications below
• ChIP: See 1 publications below

Product Information

12789-1-AP targets human BCL2 in WB, IP, IHC, IF, FC, CoIP, chIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, Goat, chicken, sheep, rabbit, fish, zebrafish, hamster, pig, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: human BCL2 fusion protein Ag3508
• Full Name: B-cell CLL/lymphoma 2
• Calculated molecular weight: 26 kDa
• Observed molecular weight: 25-30 kDa
• GenBank accession number: BC027258
• Gene symbol: BCL2
• Gene ID (NCBI): 596
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

1. What is Bcl-2?

Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.

2. FAQs for Bcl-2

 a. How to measure Bcl-2 and Bax apoptotic markers by western blotting

Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.

b. What band size should I expect in western blotting for Bcl-2?

The molecular weight of Bcl-2 is 26 kDa.

Publications

Species	Application	Title
human	WB	Signal Transduct Target Ther; Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy; Authors - Yang Meng
mouse	WB,CoIP	Cell Metab; Exercise-activated hepatic autophagy via the FN1-α5β1 integrin pathway drives metabolic benefits of exercise; Authors - Kenta Kuramoto
human	WB	ACS Nano; Nanoparticle-Mediated Co-Delivery of Notch-1 Antibodies and ABT-737 as a Potent Treatment Strategy for Triple-Negative Breast Cancer.; Authors - Danielle M Valcourt
human	WB	Mol Cancer; CircEZH2/miR-133b/IGF2BP2 aggravates colorectal cancer progression via enhancing the stability of m6A-modified CREB1 mRNA.; Authors - Bing Yao
human	WB	J Extracell Vesicles; Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway.; Authors - Xiaohan Gao
human	WB	Acta Pharm Sin B; Histone deacetylase inhibitors inhibit cervical cancer growth through Parkin acetylation-mediated mitophagy.; Authors - Xin Sun

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jia (Verified Customer) (03-01-2022)

The antibody works well for Bcl2, we recommend it.

• Applications: Western Blot

FH

Giyeong (Verified Customer) (02-18-2022)

Works well with sharp distinct vand

• Applications: Western Blot

FH

Morgan (Verified Customer) (10-26-2021)

This product worked really well. I attached photos to show how clean the blot came out when used on ovarian cancer cells (OV90s). I used 1:500 for the first use, but I could definitely use less antibody next time.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: OV90 (Ovarian cancer cell line)

human BCL2 Antibody Western Blot validation (1:500 dilution) in OV90 (Ovarian cancer cell line) (Cat no:12789-1-AP)

FH

Tom (Verified Customer) (10-09-2020)

This antibody recognizes Bcl-2 at the correct molecular weight. Highly recommended!

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Heart

FH

Bastien (Verified Customer) (08-19-2020)

Staining of B cells from mice bone marrow after cytoplasmic permezbiliation. The cells were first fixed and permeabilized with intracellular fix and perm set from ebioscience and then stained with 0,05 µl/ million cells with Bcl2 antibody (12789-1-AP) during 50min After, a seconde staining was performed with 0,1µl/million cells of F(ab')2-Donkey anti-Rabbit IgG (H+L), PE, Secondary Antibody from invitrogen. In blue secondary antibody only and in red primary (12789-1-AP) +secondary antibody.

• Applications: Flow Cytometry
• Primary Antibody Dilution: 0,05µl/10^6 cells
• Cell Tissue Type: B cells

human BCL2 Antibody Flow Cytometry validation (0,05µl/10^6 cells dilution) in B cells (Cat no:12789-1-AP)

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