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  • KD/KO Validated

human BCL2 Polyclonal antibody

human BCL2 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (9)

Applications

WB, IP, IHC, IF, FC, CoIP, chIP, ELISA

Conjugate

Unconjugated

Cat no : 12789-1-AP

Synonyms

Apoptosis regulator Bcl 2, B cell CLL/lymphoma 2, BCL2, BCL-2, Oncogene B cell leukemia 2



Tested Applications

Positive WB detected inA431 cells, HepG2 cells, Jurkat cells, HEK-293 cells, HL-60 cells, U-251 cells, U-937 cells, MCF-7 cells, U2OS cells
Positive IP detected inHL-60 cells, HepG2 cells, MCF-7 cells
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells, human peripheral blood lymphocytes

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:300
Sample-dependent, check data in validation data gallery

Product Information

12789-1-AP targets human BCL2 in WB, IP, IHC, IF, FC, CoIP, chIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, bovine, canine, chicken, fish, goat, hamster, Hu Sheep, pig, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen human BCL2 fusion protein Ag3508
Full Name B-cell CLL/lymphoma 2
Calculated molecular weight 26 kDa
Observed molecular weight 25-30 kDa
GenBank accession numberBC027258
Gene symbol BCL2
Gene ID (NCBI) 596
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

1. What is Bcl-2?

Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.

2. FAQs for Bcl-2

 a. How to measure Bcl-2 and Bax apoptotic markers by western blotting

Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.

b. What band size should I expect in western blotting for Bcl-2?

The molecular weight of Bcl-2 is 26 kDa.


Protocols

Product Specific Protocols
WB protocol for human BCL2 antibody 12789-1-APDownload protocol
IHC protocol for human BCL2 antibody 12789-1-APDownload protocol
IP protocol for human BCL2 antibody 12789-1-APDownload protocol
FC protocol for human BCL2 antibody 12789-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cancer

CircEZH2/miR-133b/IGF2BP2 aggravates colorectal cancer progression via enhancing the stability of m6A-modified CREB1 mRNA.

Authors - Bing Yao
humanWB

J Extracell Vesicles

Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway.

Authors - Xiaohan Gao
mouseWB

Nat Microbiol

The interferon-inducible isoform of NCOA7 inhibits endosome-mediated viral entry.

Authors - Tomas Doyle
humanWB

ACS Nano

PMID: 31670945

Authors - Ying Xu
humanWB

Sci Adv

Genomic gain of RRS1 promotes hepatocellular carcinoma through reducing the RPL11-MDM2-p53 signaling.

Authors - Pengbo Cao
mouseWB

Sci Adv

m6A mRNA modification maintains colonic epithelial cell homeostasis via NF-κB-mediated antiapoptotic pathway.

Authors - Ting Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Jia (Verified Customer) (03-01-2022)

The antibody works well for Bcl2, we recommend it.

  • Applications: Western Blot
FH

Giyeong (Verified Customer) (02-18-2022)

Works well with sharp distinct vand

  • Applications: Western Blot
FH

Morgan (Verified Customer) (10-26-2021)

This product worked really well. I attached photos to show how clean the blot came out when used on ovarian cancer cells (OV90s). I used 1:500 for the first use, but I could definitely use less antibody next time.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: OV90 (Ovarian cancer cell line)
human BCL2 Antibody Western Blot validation (1:500 dilution) in OV90 (Ovarian cancer cell line) (Cat no:12789-1-AP)
FH

Tom (Verified Customer) (10-09-2020)

This antibody recognizes Bcl-2 at the correct molecular weight. Highly recommended!

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Heart
FH

Bastien (Verified Customer) (08-19-2020)

Staining of B cells from mice bone marrow after cytoplasmic permezbiliation. The cells were first fixed and permeabilized with intracellular fix and perm set from ebioscience and then stained with 0,05 µl/ million cells with Bcl2 antibody (12789-1-AP) during 50min After, a seconde staining was performed with 0,1µl/million cells of F(ab')2-Donkey anti-Rabbit IgG (H+L), PE, Secondary Antibody from invitrogen. In blue secondary antibody only and in red primary (12789-1-AP) +secondary antibody.

  • Applications: Flow Cytometry
  • Primary Antibody Dilution: 0,05µl/10^6 cells
  • Cell Tissue Type: B cells
human BCL2 Antibody Flow Cytometry validation (0,05µl/10^6 cells dilution) in B cells (Cat no:12789-1-AP)