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  • KD/KO Validated

BCL2 Polyclonal antibody

BCL2 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (9)

Applications

WB, IP, IHC, IF, FC, chIP, ELISA

Conjugate

Unconjugated

Cat no : 12789-1-AP

Synonyms

Apoptosis regulator Bcl 2, B cell CLL/lymphoma 2, BCL2, BCL-2, Oncogene B cell leukemia 2



Tested Applications

Positive WB detected inHL-60 cells, HepG2 cells, Jurkat cells, HEK-293 cells, U-937 cells
Positive IP detected inHL-60 cells, HepG2 cells, MCF-7 cells
Positive IHC detected inhuman tonsillitis tissue, human breast cancer tissue, human colon cancer tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:300
Sample-dependent, check data in validation data gallery

Product Information

12789-1-AP targets BCL2 in WB, IP, IHC, IF, FC, chIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity Astragalus membranaceus, Bovine, chicken, cow, guinea pig, human, N. guentheri, pig, rabbit, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCL2 fusion protein Ag3508
Full Name B-cell CLL/lymphoma 2
Calculated molecular weight 26 kDa
Observed molecular weight 25-30 kDa
GenBank accession numberBC027258
Gene symbol BCL2
Gene ID (NCBI) 596
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

1. What is Bcl-2?

Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.

2. FAQs for Bcl-2

 a. How to measure Bcl-2 and Bax apoptotic markers by western blotting

Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.

b. What band size should I expect in western blotting for Bcl-2?

The molecular weight of Bcl-2 is 26 kDa.


Protocols

Product Specific Protocols
WB protocol for BCL2 antibody 12789-1-APDownload protocol
IHC protocol for BCL2 antibody 12789-1-APDownload protocol
IP protocol for BCL2 antibody 12789-1-APDownload protocol
FC protocol for BCL2 antibody 12789-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Onco Targets Ther

XRCC1 rs1799782 (C194T) polymorphism correlated with tumor metastasis and molecular subtypes in breast cancer.

Authors - Qing Li
humanWB

Cancer Manag Res

Pyrroline-5-carboxylate reductase 1 promotes cell proliferation via inhibiting apoptosis in human malignant melanoma.

Authors - Yingyi Ye
humanWB

J Cell Physiol

Dehydroepiandrosterone stimulates inflammation and impairs ovarian functions of polycystic ovary syndrome.

Authors - Yulin Li
humanWB

BMC Cancer

PMID: 30594176

Authors - Yue Li
mouseWB

Front Pharmacol

Protective Effects of Costunolide Against D-Galactosamine and Lipopolysaccharide-Induced Acute Liver Injury in Mice.

Authors - Jingxin Mao
mouseWB,IHC

Mediators Inflamm

The Protective Effects of Levo-Tetrahydropalmatine on ConA-Induced Liver Injury Are via TRAF6/JNK Signaling.

Authors - Qiang Yu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Bastien (Verified Customer) (08-19-2020)

Staining of B cells from mice bone marrow after cytoplasmic permezbiliation. The cells were first fixed and permeabilized with intracellular fix and perm set from ebioscience and then stained with 0,05 µl/ million cells with Bcl2 antibody (12789-1-AP) during 50min After, a seconde staining was performed with 0,1µl/million cells of F(ab')2-Donkey anti-Rabbit IgG (H+L), PE, Secondary Antibody from invitrogen. In blue secondary antibody only and in red primary (12789-1-AP) +secondary antibody.

  • Applications: Flow Cytometry
  • Primary Antibody Dilution: 0,05µl/10^6 cells
  • Cell Tissue Type: B cells
BCL2 Antibody Flow Cytometry validation (0,05µl/10^6 cells dilution) in B cells (Cat no:12789-1-AP)