Validation Data Gallery
|Positive WB detected in||HL-60 cells, HepG2 cells, Jurkat cells, HEK-293 cells, U-937 cells|
|Positive IP detected in||HL-60 cells, HepG2 cells, MCF-7 cells|
|Positive IHC detected in||human tonsillitis tissue, human breast cancer tissue, human colon cancer tissue, human lymphoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||Jurkat cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:300|
|Sample-dependent, check data in validation data gallery|
12789-1-AP targets BCL2 in WB, IP, IHC, IF, FC, chIP, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||Astragalus membranaceus, Bovine, chicken, cow, guinea pig, human, N. guentheri, pig, rabbit, sheep|
|Host / Isotype||Rabbit / IgG|
|Immunogen||BCL2 fusion protein Ag3508|
|Full Name||B-cell CLL/lymphoma 2|
|Calculated molecular weight||26 kDa|
|Observed molecular weight||25-30 kDa|
|GenBank accession number||BC027258|
|Gene ID (NCBI)||596|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
1. What is Bcl-2?
Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.
2. FAQs for Bcl-2
a. How to measure Bcl-2 and Bax apoptotic markers by western blotting
Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.
b. What band size should I expect in western blotting for Bcl-2?
The molecular weight of Bcl-2 is 26 kDa.
|Product Specific Protocols|
|WB protocol for BCL2 antibody 12789-1-AP||Download protocol|
|IHC protocol for BCL2 antibody 12789-1-AP||Download protocol|
|IP protocol for BCL2 antibody 12789-1-AP||Download protocol|
|FC protocol for BCL2 antibody 12789-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Bastien (Verified Customer) (08-19-2020)
Staining of B cells from mice bone marrow after cytoplasmic permezbiliation. The cells were first fixed and permeabilized with intracellular fix and perm set from ebioscience and then stained with 0,05 µl/ million cells with Bcl2 antibody (12789-1-AP) during 50min After, a seconde staining was performed with 0,1µl/million cells of F(ab')2-Donkey anti-Rabbit IgG (H+L), PE, Secondary Antibody from invitrogen. In blue secondary antibody only and in red primary (12789-1-AP) +secondary antibody.