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  • KD/KO Validated

GADD45A Polyclonal antibody

GADD45A Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13747-1-AP

Synonyms

DDIT 1, DDIT1, GADD45, GADD45A



Tested Applications

Positive WB detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13747-1-AP targets GADD45A in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GADD45A fusion protein Ag4687
Full Name growth arrest and DNA-damage-inducible, alpha
Calculated Molecular Weight 165 aa, 18 kDa
Observed Molecular Weight18 kDa, 33 kDa
GenBank Accession NumberBC011757
Gene Symbol GADD45A
Gene ID (NCBI) 1647
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GADD45A, also named as DDIT1 and GADD45, belongs to the GADD45 family. It binds to proliferating cell nuclear antigen. GADD45A may affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. GADD45A plays an essential role in active DNA demethylation during terminal osteogenic differentiation of adipose-derived mesenchymal stem cells. GADD45A has Dimer (~28-36 kDa) and Monomer (14-18 kDa) forms (PMID:11498536).

Protocols

Product Specific Protocols
WB protocol for GADD45A antibody 13747-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Res

DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.

Authors - Pengli Zheng
humanWB

Phytother Res

Activation of endoplasmic reticulum stress by harmine suppresses the growth of esophageal squamous cell carcinoma

Authors - Ronghui Fan
ratWB

Int J Nanomedicine

TiO2 Nanoparticles Caused DNA Damage in Lung and Extra-Pulmonary Organs Through ROS-Activated FOXO3a Signaling Pathway After Intratracheal Administration in Rats.

Authors - Bin Han
humanWB

Sci Rep

Down-regulation of GADD45A enhances chemosensitivity in melanoma.

Authors - Jia Liu
humanWB,IHC

J Exp Clin Cancer Res

Flavagline analog FL3 induces cell cycle arrest in urothelial carcinoma cell of the bladder by inhibiting the Akt/PHB interaction to activate the GADD45α pathway.

Authors - Gangjun Yuan
  • KD Validated
humanWB

J Cell Physiol

miR-124 and miR-9 Mediated Downregulation of HDAC5 Promotes Neurite Development Through Activating MEF2C- GPM6A Pathway.

Authors - Xi Gu