Anticorps Polyclonal de lapin anti-PCNA

• Phare
• Validé par KD/KO

PCNA Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (5)

Applications

WB, IP, IHC, IF, CoIP, Cell treatment, ELISA

Conjugaison

Non conjugué

Publications

805

Avis (2)

N° de cat : 10205-2-AP

Synonymes

PCNA

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• Galerie de données de validation
• Proteintech vs. Other Vendors

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  WB analysis of HEK293 cells using 10205-2-AP

  WB result of PCNA antibody (10205-2-AP, 1:10000) with si-control and si-PCNA transfected HEK293 cells.

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  WB analysis using 10205-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10205-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10205-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 10205-2-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 10205-2-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10205-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10205-2-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10205-2-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 10205-2-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10205-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 10205-2-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 10205-2-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 10205-2-AP

  IP result of anti-PCNA(IP:10205-2-AP, 4ug; Detection:10205-2-AP 1:20000) with MCF-7 cells lysate 1880 ug.

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  IP experiment using 10205-2-AP

  IP result of anti-PCNA (10205-2-AP for IP and Detection) with MCF7 cell.

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  IHC staining of human stomach cancer using 10205-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 10205-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10205-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human malignant melanoma using 10205-2-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:200 (under 10x lens)..

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  IHC staining of human malignant melanoma using 10205-2-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:200 (under 40x lens)..

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  IHC staining of human liver cancer using 10205-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10205-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10205-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10205-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10205-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10205-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 10205-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10205-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10205-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PCNA antibody (10205-2-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL94-phalloidin (red), DAPI (Blue).

• 

  IF Staining of HepG2 using 10205-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10205-2-AP (PCNA antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse testis using 10205-2-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PCNA antibody (10205-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 10205-2-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PCNA antibody (10205-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of Neuro-2a using 10205-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using 10205-2-AP (PCNA antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human breast cancer using 10205-2-AP

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 10205-2-AP (PCNA antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Jurkat using 10205-2-PBS

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human PCNA (10205-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), and 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules C2C12, cellules HEK293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules MCF-7, cellules NIH/3T3, cellules PC-12, cellules Raji, tissu hépatique de rat, tissu hépatique de souris, tissu splénique de souris, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IP: cellules MCF-7, N/A
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu de cancer du côlon humain, tissu de cancer du foie humain, tissu de cancer du sein humain, tissu de mélanome malin humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2, cellules MCF-7, cellules Neuro-2a, tissu de cancer du sein humain, tissu testiculaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1500-1:6000
• Immunofluorescence (IF): IF : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 597 publications below
• IHC: See 217 publications below
• IF: See 74 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

10205-2-AP cible PCNA dans les applications de WB, IP, IHC, IF, CoIP, Cell treatment, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Chèvre, Humain, Lapin, poulet, souris, embryons de médaka, fish
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PCNA Protéine recombinante Ag0277
• Nom complet: proliferating cell nuclear antigen
• Masse moléculaire calculée: 29 kDa/31 kDa
• Poids moléculaire observé: 36-38 kDa
• Numéro d’acquisition GenBank: BC000491
• Symbole du gène: PCNA
• Identification du gène (NCBI): 5111
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. This antibody is a rabbit polyclonal antibody raised against an internal region of human PCNA. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa (PMID: 1358458).

Publications

Species	Application	Title
human	WB	Mil Med Res; Aspartoacylase suppresses prostate cancer progression by blocking LYN activation; Authors - Hong Weng
mouse	IF	Brain Behav Immun; An enriched environment restores hepatitis B vaccination-mediated impairments in synaptic function through IFN-γ/Arginase1 signaling.; Authors - Fangfang Qi
rabbit	IF	Nat Commun; Four-dimensional hydrogel dressing adaptable to the urethral microenvironment for scarless urethral reconstruction; Authors - Yujie Hua
human	WB	Nat Commun; The RNA-binding protein LRPPRC promotes resistance to CDK4/6 inhibition in lung cancer; Authors - Wei Zhou
rat	WB	Acta Pharm Sin B; Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2.; Authors - Xunxun Wu
mouse	WB	Hepatology; Liver-specific deficiency of unc-51 like kinase 1 and 2 protects mice from acetaminophen-induced liver injury.; Authors - Yu Sun

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Mi (Verified Customer) (06-25-2023)

It works well in human brown adipocyte cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human brown adipocytes

FH

Emma (Verified Customer) (11-29-2021)

Nice antibody which worked well on PC3 cells fixed with 4% PFA. Antibody used at 1:50

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: PC3 cells

PCNA Antibody Immunofluorescence validation (1:50 dilution) in PC3 cells (Cat no:10205-2-AP)

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