FEN1 Polyclonal antibody

FEN1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14768-1-AP

Synonyms

DNase IV, FEN 1, FEN1, Flap endonuclease 1, flap structure endonuclease 1, hFEN 1, Maturation factor 1, MF1, RAD2



Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells
Positive IP detected inNIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14768-1-AP targets FEN1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FEN1 fusion protein Ag6552
Full Name flap structure-specific endonuclease 1
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC000323
Gene Symbol FEN1
Gene ID (NCBI) 2237
RRIDAB_2102780
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FEN1(Flap endonuclease-1) is the prototypical member of the 5'-nuclease superfamily, whose activities span a range of cellular pathways involved in DNA replication and genome maintenance (PMID: 22118811, 21496641, 20929870). FEN1 is a structure-selective metallonuclease essential for Okazaki fragment maturation through efficient removal of 5' flaps resulting from strand displacement during lagging-strand synthesis (PMID: 8144677, 9081985). FEN1 is overexpressed in multiple cancer types, and has been suggested both as a biomarker relating to prognosis and disease progression and as a potential therapeutic target (PMID: 19010819, 16879693, 19596913, 27526030).

Protocols

Product Specific Protocols
WB protocol for FEN1 antibody 14768-1-APDownload protocol
IHC protocol for FEN1 antibody 14768-1-APDownload protocol
IF protocol for FEN1 antibody 14768-1-APDownload protocol
IP protocol for FEN1 antibody 14768-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Nat Commun

Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration.

Authors - Megha Jhanji
mouseWB

J Med Chem

CJ2: A Novel Potent Platinum(IV) Prodrug Enhances Chemo-Immunotherapy by Facilitating PD-L1 Degradation in the Cytoplasm and Cytomembrane

Authors - Renming Fan
humanWB

J Mol Cell Biol

SUMO-1 modification of FEN1 facilitates its interaction with Rad9-Rad1-Hus1 to counteract DNA replication stress.

Authors - Xiaoli Xu
humanWB

Biology (Basel)

In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.

Authors - Olga Gourdomichali
humanWB

Am J Physiol Cell Physiol

Succinylation at a key residue of FEN1 is involved in the DNA damage response to maintain genome stability.

Authors - Rongyi Shi
humanWB,IHC

Dis Markers

Upregulation of FEN1 Is Associated with the Tumor Progression and Prognosis of Hepatocellular Carcinoma.

Authors - Yingying Zhang