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  • KD/KO Validated

POLD3 Polyclonal antibody

POLD3 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 21935-1-AP

Synonyms

DNA polymerase delta subunit 3, KIAA0039, P66, P68, POLD3



Tested Applications

Positive WB detected inMCF-7 cells, mouse spleen tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman cervical cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21935-1-AP is POLD3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLD3 fusion protein Ag16570
Full Name polymerase (DNA-directed), delta 3, accessory subunit
Calculated molecular weight 466 aa, 51 kDa
Observed molecular weight 51-70 kDa
GenBank accession numberBC108908
Gene symbol POLD3
Gene ID (NCBI) 10714
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for POLD3 antibody 21935-1-APDownload protocol
IHC protocol for POLD3 antibody 21935-1-APDownload protocol
IF protocol for POLD3 antibody 21935-1-APDownload protocol
IP protocol for POLD3 antibody 21935-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Genes Dev

Break-induced replication promotes fragile telomere formation.

Authors - Zhe Yang
  • KO Validated