The advantages of CoraLite direct staining for IF

Video highlights of how CoraLite® antibodies can streamline your multiplex immunofluorescence staining.

This seminar was originally recorded on May 11, 2020 as part of the MetroFlow group’s corporate member seminar series. Dr. William Olds, Scientific Officer at Proteintech, highlights how CoraLite® antibodies can streamline your multiplex immunofluorescence staining.
Life After FC: Using CoraLite fluorescent dye-conjugated antibodies to simplify your multiplex imaging by Will Olds, PhD - Scientific Officer


CoraLite Fluorescent Dye-Conjugated Antibodies

Proteintech's popular monoclonal and polyclonal antibodies are now available directly conjugated with fluorescent dyes:  CoraLite488,  CoraLite594,  and CoraLite647. They act as perfect tools for immunofluorescence studies requiring multiplex co-labeling studies without the need for secondary antibodies:

  • Can be used directly in immunofluorescence studies without the need for secondary antibodies

  • Offer bright and long-lasting fluorescence

  • Can be multiplexed with other CoraLite dyes and popular dyes (e.g. nuclear stains, DAPI, etc.)

CoraLite conjugated antibodies

Immunofluorescent analysis of ( 4% PFA) fixed rat brain tissue using 17490-1-AP (MAP2 antibody) at dilution of 1:100 and CoraLite594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 60190-1-Ig (GFAP antibody, green).
Immunofluorescent analysis of ( -20°C Ethanol) fixed oleic acid treated HepG2 cells using 15294-1-AP (ADRP/Perilipin 2 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Red: CL555-phalloidin staining of F-actin.

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