ST2 Polyclonal antibody

ST2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11920-1-AP

Synonyms

DER4, FIT 1, IL1RL1, IL-1RL1, IL-33R, interleukin 1 receptor like 1, Protein ST2, ST2, ST2L, ST2V, T1



Tested Applications

Positive WB detected inhuman placenta tissue, mouse lung tissue, rat lung tissue
Positive IHC detected inmouse heart tissue, human lung cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11920-1-AP targets ST2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ST2 fusion protein Ag2555
Full Name interleukin 1 receptor-like 1
Calculated Molecular Weight 63 kDa, 37 kDa, 30 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC030975
Gene Symbol IL1RL1
Gene ID (NCBI) 9173
RRIDAB_906359
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ST2, also known as IL1RL1, is a member of the IL-1 superfamily and serves as the receptor for IL-33. It plays a major role in immune and inflammatory responses. ST2 is expressed by various types of immune cells including Th2 cells, mast cells, type 2 innate lymphoid cells, eosinophils, basophils, NK cells, and non-immune cells, including cardiomyocytes. Two forms of ST2 exist: a membrane-bound form and a soluble form (sST2). The membrane-bound form activates the MyD88/NF-κB signaling pathway to enhance mast cell, Th2, regulatory T cell (Treg), and innate lymphoid cell type 2 functions. sST2 acts as a decoy receptor. (PMID: 27055914; 23999434; 28484466)

Protocols

Product Specific Protocols
WB protocol for ST2 antibody 11920-1-APDownload protocol
IHC protocol for ST2 antibody 11920-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF,WB

Adv Healthc Mater

Ultrasmall Nanoparticles Regulate Immune Microenvironment by Activating IL-33/ST2 to Alleviate Renal Ischemia-Reperfusion Injury

Authors - Liyao Xu
humanIHC

Proc Natl Acad Sci U S A

IL-33 activates tumor stroma to promote intestinal polyposis.

Authors - Rebecca L Maywald
humanIHC,IF

J Invest Dermatol

Increased Type 2 Innate Lymphoid Cells in Patients with Drug Reaction with Eosinophilia and Systemic Symptoms Syndrome.

Authors - Yi-Giien Tsai
humanWB,IF

Oncogene

The reciprocal interaction between tumor cells and activated fibroblasts mediated by TNF-α/IL-33/ST2L signaling promotes gastric cancer metastasis.

Authors - Quan Zhou
human,mouseWB,IHC

Am J Pathol

Cross Talk between ST2 and Transforming Growth Factor-β Receptor Signaling Promotes Renal Fibrosis

Authors - Xingxing Zhu
rat,mouseWB,IHC,IF

J Neuroinflammation

Red nucleus IL-33 facilitates the early development of mononeuropathic pain in male rats by inducing TNF-α through activating ERK, p38 MAPK, and JAK2/STAT3.

Authors - Hao-Nan Li