IL-33 Monoclonal antibody

IL-33 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgM

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2A9C7

Cat no : 66235-1-Ig

Synonyms

C9orf26, DVS27, IL 1F11, IL 33, IL1F11, IL33, IL-33, Interleukin 1 family member 11, interleukin 33, NF HEV, NFEHEV, NFHEV, RP11 575C20.2



Tested Applications

Positive WB detected inTHP-1 cells, hTERT-RPE1 cells, human lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

66235-1-Ig targets IL-33 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen IL-33 fusion protein Ag21430
Full Name interleukin 33
Calculated molecular weight 30 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC047085
Gene symbol IL33
Gene ID (NCBI) 90865
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL-33 is an IL-1 family cytokine and nuclear alarmin, and is constitutively expressed in epithelial barrier tissues and human blood vessels. IL-33 is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. It also acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury.

Protocols

Product Specific Protocols
WB protocol for IL-33 antibody 66235-1-IgDownload protocol
IHC protocol for IL-33 antibody 66235-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Oncol

Cancer-associated fibroblasts educate normal fibroblasts to facilitate cancer cell spreading and T cell suppression.

Authors - Go Itoh
humanIHC

Cell Cycle

Roles of Clara cell 10-kD protein and type 2 innate lymphoid cells in allergic rhinitis.

Authors - Xiaobo Long
humanWB,IHC

Sci Rep

Dysfunctional EAT thickness may promote maladaptive heart remodeling in CVD patients through the ST2-IL33 system, directly related to EPAC protein expression.

Authors - Elena Vianello
humanWB,IF

Cells

Helicobacter pylori Induces IL-33 Production and Recruits ST-2 to Lipid Rafts to Exacerbate Inflammation.

Authors - Chia-Jung Kuo
mouseWB,IF

Int J Mol Sci

An Amide Alkaloid Isolated from Ephedra sinica Ameliorates OVA-Induced Allergic Asthma by Inhibiting Mast Cell Activation and Dendritic Cell Maturation

Authors - Jufang Jia
humanWB,IHC,IF

Cell Death Dis

Macrophages originated IL-33/ST2 inhibits ferroptosis in endometriosis via the ATF3/SLC7A11 axis

Authors - Qiong Wu