IL-33 Monoclonal antibody

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  WB analysis of human lung using 66235-1-Ig

  human lung tissue were subjected to SDS PAGE followed by western blot with 66235-1-Ig (IL33 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of THP-1 using 66235-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66235-1-Ig (IL-33 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of hTERT-RPE1 using 66235-1-Ig

  hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 66235-1-Ig (IL-33 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 66235-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66235-1-Ig (IL-33 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 66235-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66235-1-Ig (IL-33 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IL-33 Monoclonal Antibody for WB, ELISA

Cat No. 66235-1-Ig

Clone No.2A9C7

Publications(10)

Host / Isotype

Mouse / IgM

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

C9orf26, DVS27, IL 1F11, IL 33, IL1F11, IL33, IL-33, Interleukin 1 family member 11, interleukin 33, NF HEV, NFEHEV, NFHEV, RP11 575C20.2

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Tested Applications

• Positive WB detected in: THP-1 cells, hTERT-RPE1 cells, human lung tissue

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 4 publications below
• IF: See 5 publications below

Product Information

66235-1-Ig targets IL-33 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgM
• Class: Monoclonal
• Type: Antibody
• Immunogen: IL-33 fusion protein Ag21430
• Full Name: interleukin 33
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC047085
• Gene Symbol: IL-33
• Gene ID (NCBI): 90865
• RRID: AB_2881624
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O95760
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL-33 is an IL-1 family cytokine and nuclear alarmin, and is constitutively expressed in epithelial barrier tissues and human blood vessels. IL-33 is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. It also acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury.

Publications

Species	Application	Title
human	WB	Mol Oncol; Cancer-associated fibroblasts educate normal fibroblasts to facilitate cancer cell spreading and T cell suppression.; Authors - Go Itoh
human	IHC	Cell Cycle; Roles of Clara cell 10-kD protein and type 2 innate lymphoid cells in allergic rhinitis.; Authors - Xiaobo Long
human	WB,IHC	Sci Rep; Dysfunctional EAT thickness may promote maladaptive heart remodeling in CVD patients through the ST2-IL33 system, directly related to EPAC protein expression.; Authors - Elena Vianello
human	WB,IF	Cells; Helicobacter pylori Induces IL-33 Production and Recruits ST-2 to Lipid Rafts to Exacerbate Inflammation.; Authors - Chia-Jung Kuo
mouse	WB,IF	Int J Mol Sci; An Amide Alkaloid Isolated from Ephedra sinica Ameliorates OVA-Induced Allergic Asthma by Inhibiting Mast Cell Activation and Dendritic Cell Maturation; Authors - Jufang Jia
human	WB,IHC,IF	Cell Death Dis; Macrophages originated IL-33/ST2 inhibits ferroptosis in endometriosis via the ATF3/SLC7A11 axis; Authors - Qiong Wu