Tested Applications
| Positive WB detected in | Jurkat cells, K-562 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human lung cancer tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18221-1-AP targets IRAK4 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12982 Product name: Recombinant human IRAK4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-200 aa of BC013316 Sequence: MNKPITPSTYVRCLNVGLIRKLSDFIDPQEGWKKLAVAIKKPSGDDRYNQFHIRRFEALLQTGKSPTSELLFDWGTTNCTVGDLVDLLIQNEFFAPASLLLPDAVPKTANTLPSKEAITVQQKQMPFCDKDRTLMTPVQNLEQSYMPPDSSSPENKSLEVSDTRFHSFSFYELKNVTNNFDERPISVGGNKMGEGGFGVV Predict reactive species |
| Full Name | interleukin-1 receptor-associated kinase 4 |
| Calculated Molecular Weight | 52 kDa |
| Observed Molecular Weight | 52 kDa |
| GenBank Accession Number | BC013316 |
| Gene Symbol | IRAK4 |
| Gene ID (NCBI) | 51135 |
| RRID | AB_2878519 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NWZ3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for IRAK4 antibody 18221-1-AP | Download protocol |
| IHC protocol for IRAK4 antibody 18221-1-AP | Download protocol |
| IP protocol for IRAK4 antibody 18221-1-AP | Download protocol |
| WB protocol for IRAK4 antibody 18221-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The IRAK4 Polyclonal Antibody (Cat# 18221-1-AP) has accumulated 12 citations. These appear in journals including Cell Reports in Medicine, Cancer Letters, International Journal of Biological Sciences, Journal of Medicinal Chemistry, International Journal of Molecular Sciences, International Immunopharmacology, and Frontiers in Pharmacology. Associated research fields encompass cancer biology (hepatobiliary, pancreatic, and head/neck squamous cell carcinoma), autoimmune and inflammatory diseases (arthritis), neurobiology (anesthesia-induced cognitive impairment), ophthalmology (HSV-1 corneal infection), and pharmacology. Cited applications include WB, IHC, IF, and IP.
| Species | Application | Title |
|---|---|---|
Cell Rep Med Integrated characterization of hepatobiliary tumor organoids provides a potential landscape of pharmacogenomic interactions | ||
Cancer Lett IL-1α facilitates GSH synthesis to counteract oxidative stress in oral squamous cell carcinoma under glucose-deprivation | ||
Int J Biol Sci Targeting IL-1α Sensitizes HNSCC to PDT by Reversing Hypoxia and NF-κB-Driven Oxidative Stress Resistance. | ||
Int J Biol Sci BAP1 Represses Sequential Activation of IRAKs and NF-κB Signaling in Pancreatic Cancer | ||
J Med Chem Design, Synthesis, and Evaluation of Nucleolin-Bridged, MDM2-Recruiting IRAK4 Degraders for the Treatment of Autoimmune Diseases. | ||
Int Immunopharmacol Herbal compound cepharanthine attenuates inflammatory arthritis by blocking macrophage M1 polarization |
