ST2 Monoclonal antibody

ST2 Monoclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

6

CloneNo.

7A2A7

Cat no : 60112-1-Ig

Synonyms

DER4, FIT 1, IL1RL1, interleukin 1 receptor like 1, Protein ST2, ST2, ST2L, ST2V, T1

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Validation Data Gallery

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  WB analysis using 60112-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60112-1-Ig (ST2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SGC-7901 using 60112-1-Ig

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 60112-1-Ig (ST2 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 60112-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 60112-1-Ig (ST2 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 60112-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 60112-1-Ig (ST2 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 60112-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60112-1-Ig (ST2 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 60112-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60112-1-Ig (ST2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 60112-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60112-1-Ig (ST2 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 60112-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60112-1-Ig (ST2 antibody at dilution of 1:200 (under 40x lens).

• 

  FC experiment of HEK-293 using 60112-1-Ig

  1X10^6 HEK-293 cells were stained with 0.20ug ST2 antibody (60112-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.

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Tested Applications

• Positive WB detected in: HEK-293 cells, fetal human brain tissue, SGC-7901 cells, pig brain tissue, mouse brain tissue, HSC-T6 cells, ROS1728 cells, PC-12 cells, rat brain tissue, rat cerebellum tissue
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: HEK-293 cells

Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Product Information

60112-1-Ig targets ST2 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ST2 fusion protein Ag2555
• Full Name: interleukin 1 receptor-like 1
• Calculated molecular weight: 63 kDa, 37 kDa, 30 kDa
• Observed molecular weight: 63 kDa
• GenBank accession number: BC030975
• Gene symbol: IL1RL1
• Gene ID (NCBI): 9173
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Caprylic acid/ammonium sulfate precipitation
• Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
human		JCI Insight; Epithelial IL-33 appropriates exosome trafficking for secretion in chronic airway disease.; Authors - Ella Katz-Kiriakos
pig	IHC	Am J Physiol Renal Physiol; INTRA-RENAL MODULATION OF NF-κB ACTIVITY ATTENUATES CARDIAC INJURY IN A SWINE MODEL OF CKD: A RENAL-CARDIO AXIS.; Authors - Alejandro R Chade
human	IF, WB	Inflamm Res; Molecular hydrogen alleviates asthma through inhibiting IL-33/ILC2 axis.; Authors - Jingxi Zhang
human	IF	Sci Rep; Fatty acid-binding protein 5 limits ILC2-mediated allergic lung inflammation in a murine asthma model.; Authors - Shuhei Kobayashi
human	WB,IHC	Sci Rep; Dysfunctional EAT thickness may promote maladaptive heart remodeling in CVD patients through the ST2-IL33 system, directly related to EPAC protein expression.; Authors - Elena Vianello
mouse	IF	Immunity; Interleukin-33 Promotes Serotonin Release from Enterochromaffin Cells for Intestinal Homeostasis.; Authors - Zuojia Chen

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