ST2 Monoclonal antibody
ST2 Monoclonal Antibody for FC, IHC, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
7A2A7
Cat no : 60112-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, fetal human brain tissue, SGC-7901 cells, pig brain tissue, mouse brain tissue, HSC-T6 cells, ROS1728 cells, PC-12 cells, rat brain tissue, rat cerebellum tissue |
Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC detected in | HEK-293 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 2 publications below |
IHC | See 2 publications below |
IF | See 3 publications below |
Product Information
60112-1-Ig targets ST2 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human, mouse, pig |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | ST2 fusion protein Ag2555 |
Full Name | interleukin 1 receptor-like 1 |
Calculated molecular weight | 63 kDa, 37 kDa, 30 kDa |
Observed molecular weight | 63 kDa |
GenBank accession number | BC030975 |
Gene symbol | IL1RL1 |
Gene ID (NCBI) | 9173 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Caprylic acid/ammonium sulfate precipitation |
Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for ST2 antibody 60112-1-Ig | Download protocol |
IHC protocol for ST2 antibody 60112-1-Ig | Download protocol |
FC protocol for ST2 antibody 60112-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
JCI Insight Epithelial IL-33 appropriates exosome trafficking for secretion in chronic airway disease. | ||
Am J Physiol Renal Physiol INTRA-RENAL MODULATION OF NF-κB ACTIVITY ATTENUATES CARDIAC INJURY IN A SWINE MODEL OF CKD: A RENAL-CARDIO AXIS. | ||
Sci Rep Fatty acid-binding protein 5 limits ILC2-mediated allergic lung inflammation in a murine asthma model. | ||
Sci Rep Dysfunctional EAT thickness may promote maladaptive heart remodeling in CVD patients through the ST2-IL33 system, directly related to EPAC protein expression. | ||
Immunity Interleukin-33 Promotes Serotonin Release from Enterochromaffin Cells for Intestinal Homeostasis. |