ST2 Monoclonal antibody
ST2 Monoclonal Antibody for FC, IHC, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
7A2A7
Cat no : 60112-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, fetal human brain tissue, SGC-7901 cells, pig brain tissue, mouse brain tissue, HSC-T6 cells, ROS1728 cells, PC-12 cells, rat brain tissue, rat cerebellum tissue |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | HEK-293 cells |
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 2 publications below |
| IHC | See 2 publications below |
| IF | See 3 publications below |
Product Information
60112-1-Ig targets ST2 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | ST2 fusion protein Ag2555 |
| Full Name | interleukin 1 receptor-like 1 |
| Calculated molecular weight | 63 kDa, 37 kDa, 30 kDa |
| Observed molecular weight | 63 kDa |
| GenBank accession number | BC030975 |
| Gene symbol | IL1RL1 |
| Gene ID (NCBI) | 9173 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Caprylic acid/ammonium sulfate precipitation |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ST2 antibody 60112-1-Ig | Download protocol |
| IHC protocol for ST2 antibody 60112-1-Ig | Download protocol |
| FC protocol for ST2 antibody 60112-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
JCI Insight Epithelial IL-33 appropriates exosome trafficking for secretion in chronic airway disease. | ||
Am J Physiol Renal Physiol INTRA-RENAL MODULATION OF NF-κB ACTIVITY ATTENUATES CARDIAC INJURY IN A SWINE MODEL OF CKD: A RENAL-CARDIO AXIS. | ||
Sci Rep Fatty acid-binding protein 5 limits ILC2-mediated allergic lung inflammation in a murine asthma model. | ||
Sci Rep Dysfunctional EAT thickness may promote maladaptive heart remodeling in CVD patients through the ST2-IL33 system, directly related to EPAC protein expression. | ||
Immunity Interleukin-33 Promotes Serotonin Release from Enterochromaffin Cells for Intestinal Homeostasis. |
