Validation Data Gallery
|Positive WB detected in||HEK-293 cells, fetal human brain tissue, SGC-7901 cells, pig brain tissue, mouse brain tissue, HSC-T6 cells, ROS1728 cells, PC-12 cells, rat brain tissue, rat cerebellum tissue|
|Positive IHC detected in||human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Sample-dependent, check data in validation data gallery|
60112-1-Ig targets ST2 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
|Tested Reactivity||human, mouse, rat, pig|
|Cited Reactivity||human, mouse, pig|
|Host / Isotype||Mouse / IgG1|
|Immunogen||ST2 fusion protein Ag2555|
|Full Name||interleukin 1 receptor-like 1|
|Calculated molecular weight||63 kDa, 37 kDa, 30 kDa|
|Observed molecular weight||63 kDa|
|GenBank accession number||BC030975|
|Gene ID (NCBI)||9173|
|Purification Method||Caprylic acid/ammonium sulfate precipitation|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
ST2, also known as IL1RL1, is a member of the IL-1 superfamily and serves as the receptor for IL-33. It plays a major role in immune and inflammatory responses. ST2 is expressed by various types of immune cells including Th2 cells, mast cells, type 2 innate lymphoid cells, eosinophils, basophils, NK cells, and non-immune cells, including cardiomyocytes. Two forms of ST2 exist: a membrane-bound form and a soluble form (sST2). The membrane-bound form activates the MyD88/NF-κB signaling pathway to enhance mast cell, Th2, regulatory T cell (Treg), and innate lymphoid cell type 2 functions. sST2 acts as a decoy receptor. (PMID: 27055914; 23999434; 28484466)
Interleukin-33 Promotes Serotonin Release from Enterochromaffin Cells for Intestinal Homeostasis.
Dysfunctional EAT thickness may promote maladaptive heart remodeling in CVD patients through the ST2-IL33 system, directly related to EPAC protein expression.
Fatty acid-binding protein 5 limits ILC2-mediated allergic lung inflammation in a murine asthma model.
Targeting IL-17alpha to promote anti-PD-1 therapy effect by screening the tumor immune microenvironment in a mouse oral carcinogenesis model
Molecular hydrogen alleviates asthma through inhibiting IL-33/ILC2 axis.
Am J Physiol Renal Physiol
INTRA-RENAL MODULATION OF NF-κB ACTIVITY ATTENUATES CARDIAC INJURY IN A SWINE MODEL OF CKD: A RENAL-CARDIO AXIS.