IL-1RL1/ST2 Monoclonal antibody

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  WB analysis using 60112-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60112-1-Ig (ST2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 60112-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 60112-1-Ig (ST2 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 60112-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60112-1-Ig (ST2 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 60112-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60112-1-Ig (ST2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 60112-1-Ig

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 60112-1-Ig (ST2 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 60112-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 60112-1-Ig (ST2 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

IL-1RL1/ST2 Monoclonal Antibody for WB, ELISA

Cat No. 60112-1-Ig

Clone No.7A2A7

Publications(18)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, ELISA

Synonyms

IL1RL1, 7A2A7, DER4, EC:3.2.2.6, FIT 1

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Tested Applications

• Positive WB detected in: HEK-293 cells, fetal human brain tissue, SGC-7901 cells, pig brain tissue, mouse brain tissue, HSC-T6 cells, ROS1728 cells, PC-12 cells, rat brain tissue, rat cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below

Product Information

60112-1-Ig targets IL-1RL1/ST2 in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: IL-1RL1/ST2 fusion protein Ag2555
• Full Name: interleukin 1 receptor-like 1
• Calculated Molecular Weight: 63 kDa, 37 kDa, 30 kDa
• Observed Molecular Weight: 63 kDa
• GenBank Accession Number: BC030975
• Gene Symbol: IL1RL1
• Gene ID (NCBI): 9173
• RRID: AB_2264785
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q01638
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ST2, also known as IL1RL1, is a member of the IL-1 superfamily and serves as the receptor for IL-33. It plays a major role in immune and inflammatory responses. ST2 is expressed by various types of immune cells including Th2 cells, mast cells, type 2 innate lymphoid cells, eosinophils, basophils, NK cells, and non-immune cells, including cardiomyocytes. Two forms of ST2 exist: a membrane-bound form and a soluble form (sST2). The membrane-bound form activates the MyD88/NF-κB signaling pathway to enhance mast cell, Th2, regulatory T cell (Treg), and innate lymphoid cell type 2 functions. sST2 acts as a decoy receptor. (PMID: 27055914; 23999434; 28484466)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse		Immunity; Interleukin-33 Promotes Serotonin Release from Enterochromaffin Cells for Intestinal Homeostasis.; Authors - Zuojia Chen
mouse		Brain Behav Immun; Fibroblast-derived IL-33 exacerbates orofacial neuropathic pain via the activation of TRPA1 in trigeminal ganglion neurons; Authors - Yousuke Ikehata
human		Neurology; Association of Soluble ST2 With Functional Outcome, Perihematomal Edema, and Immune Response After Intraparenchymal Hemorrhage; Authors - Matthew B Bevers
human	WB	Sci Rep; Dysfunctional EAT thickness may promote maladaptive heart remodeling in CVD patients through the ST2-IL33 system, directly related to EPAC protein expression.; Authors - Elena Vianello
human		Sci Rep; Fatty acid-binding protein 5 limits ILC2-mediated allergic lung inflammation in a murine asthma model.; Authors - Shuhei Kobayashi
mouse		Cancer Biomark; Targeting IL-17alpha to promote anti-PD-1 therapy effect by screening the tumor immune microenvironment in a mouse oral carcinogenesis model; Authors - Shan Wang