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IL-33 Polyclonal antibody

IL-33 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human and More (2)





Cat no : 12372-1-AP


C9orf26, DVS27, IL 1F11, IL 33, IL1F11, IL33, IL-33, Interleukin 1 family member 11, interleukin 33, NF HEV, NFEHEV, NFHEV, RP11 575C20.2

Tested Applications

Positive WB detected inPMA, LPS and Brefeldin A treated THP-1 cells, Recombinant protein
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

12372-1-AP targets IL-33 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-33 fusion protein Ag2957
Full Name interleukin 33
Calculated molecular weight 30 kDa
GenBank accession numberBC047085
Gene symbol IL33
Gene ID (NCBI) 90865
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL-33 is an IL-1 family cytokine and nuclear alarmin, is constitutively expressed in epithelial barrier tissues and human blood vessels. IL-33 is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. It also acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury.


Product Specific Protocols
WB protocol for IL-33 antibody 12372-1-APDownload protocol
IHC protocol for IL-33 antibody 12372-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Invest Dermatol

Increased Type 2 Innate Lymphoid Cells in Patients with Drug Reaction with Eosinophilia and Systemic Symptoms Syndrome.

Authors - Yi-Giien Tsai

Front Immunol

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Am J Pathol

Cross Talk between ST2 and Transforming Growth Factor-β Receptor Signaling Promotes Renal Fibrosis

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Free fatty acid receptor 4 deletion attenuates colitis by modulating Treg Cells via ZBED6-IL33 pathway.

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Int Immunopharmacol

Hypoxia induces the production of epithelial-derived cytokines in eosinophilic chronic rhinosinusitis with nasal polyps

Authors - Meiping Zhang

Front Microbiol

Akkermansia muciniphila Alters Gut Microbiota and Immune System to Improve Cardiovascular Diseases in Murine Model.

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