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IL-33 Polyclonal antibody

IL-33 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12372-1-AP

Synonyms

C9orf26, DVS27, IL 1F11, IL 33, IL1F11, IL33, IL-33, Interleukin 1 family member 11, interleukin 33, NF HEV, NFEHEV, NFHEV, RP11 575C20.2



Tested Applications

Positive WB detected inPMA, LPS and Brefeldin A treated THP-1 cells, Recombinant protein
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

12372-1-AP targets IL-33 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-33 fusion protein Ag2957
Full Name interleukin 33
Calculated molecular weight 30 kDa
GenBank accession numberBC047085
Gene symbol IL33
Gene ID (NCBI) 90865
RRIDAB_2877852
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL-33 is an IL-1 family cytokine and nuclear alarmin, is constitutively expressed in epithelial barrier tissues and human blood vessels. IL-33 is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. It also acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury.

Protocols

Product Specific Protocols
WB protocol for IL-33 antibody 12372-1-APDownload protocol
IHC protocol for IL-33 antibody 12372-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,IF

J Invest Dermatol

Increased Type 2 Innate Lymphoid Cells in Patients with Drug Reaction with Eosinophilia and Systemic Symptoms Syndrome.

Authors - Yi-Giien Tsai
ratWB

Front Immunol

Emodin Alleviates Sodium Taurocholate-Induced Pancreatic Acinar Cell Injury via MicroRNA-30a-5p-Mediated Inhibition of High-Temperature Requirement A/Transforming Growth Factor Beta 1 Inflammatory Signaling.

Authors - Hong Xiang
human,mouseWB,IHC

Am J Pathol

Cross Talk between ST2 and Transforming Growth Factor-β Receptor Signaling Promotes Renal Fibrosis

Authors - Xingxing Zhu
humanWB

EBioMedicine

Free fatty acid receptor 4 deletion attenuates colitis by modulating Treg Cells via ZBED6-IL33 pathway.

Authors - Shenglong Zhu
human,mouseIHC

Int Immunopharmacol

Hypoxia induces the production of epithelial-derived cytokines in eosinophilic chronic rhinosinusitis with nasal polyps

Authors - Meiping Zhang
mouseIHC

Front Microbiol

Akkermansia muciniphila Alters Gut Microbiota and Immune System to Improve Cardiovascular Diseases in Murine Model.

Authors - Xin He