IL-33 Polyclonal antibody

• 

  WB analysis using 12372-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12372-1-AP (IL-33 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12372-1-AP

  PMA, LPS, and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 12372-1-AP (IL-33 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Recombinant protein using 12372-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 12372-1-AP (IL33 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 12372-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12372-1-AP (IL-33 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 12372-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12372-1-AP (IL-33 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

IL-33 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12372-1-AP

Publications(35)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Synonyms

IL33, IL 33, IL 1F11, C9orf26, 99-270

---

Tested Applications

• Positive WB detected in: HUVEC cells, Recombinant protein, PMA, LPS and Brefeldin A treated THP-1 cells, HeLa cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 21 publications below
• IHC: See 20 publications below
• IF: See 9 publications below
• ELISA: See 2 publications below

Product Information

12372-1-AP targets IL-33 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IL-33 fusion protein Ag2957
• Full Name: interleukin 33
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC047085
• Gene Symbol: IL-33
• Gene ID (NCBI): 90865
• RRID: AB_2877852
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95760
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL-33 is an IL-1 family cytokine and nuclear alarmin, is constitutively expressed in epithelial barrier tissues and human blood vessels. IL-33 is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. It also acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury.

Publications

Species	Application	Title
mouse	WB	Adv Healthc Mater; Ultrasmall Nanoparticles Regulate Immune Microenvironment by Activating IL-33/ST2 to Alleviate Renal Ischemia-Reperfusion Injury; Authors - Liyao Xu
human	IHC,IF	J Invest Dermatol; Increased Type 2 Innate Lymphoid Cells in Patients with Drug Reaction with Eosinophilia and Systemic Symptoms Syndrome.; Authors - Yi-Giien Tsai
rat	WB	Front Immunol; Emodin Alleviates Sodium Taurocholate-Induced Pancreatic Acinar Cell Injury via MicroRNA-30a-5p-Mediated Inhibition of High-Temperature Requirement A/Transforming Growth Factor Beta 1 Inflammatory Signaling.; Authors - Hong Xiang
human,mouse	WB,IHC	Am J Pathol; Cross Talk between ST2 and Transforming Growth Factor-β Receptor Signaling Promotes Renal Fibrosis; Authors - Xingxing Zhu
human	WB	EBioMedicine; Free fatty acid receptor 4 deletion attenuates colitis by modulating Treg Cells via ZBED6-IL33 pathway.; Authors - Shenglong Zhu
human,mouse	IHC	Int Immunopharmacol; Hypoxia induces the production of epithelial-derived cytokines in eosinophilic chronic rhinosinusitis with nasal polyps; Authors - Meiping Zhang