IL-33 Polyclonal antibody

• Featured Product
• KD/KO Validated

IL-33 Polyclonal Antibody for IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

19

Cat no : 12372-1-AP

Synonyms

C9orf26, DVS27, IL 1F11, IL 33, IL1F11, IL33, IL-33, Interleukin 1 family member 11, interleukin 33, NF HEV, NFEHEV, NFHEV, RP11 575C20.2

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Validation Data Gallery

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  WB analysis of Recombinant protein using 12372-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 12372-1-AP (IL33 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 12372-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12372-1-AP (IL33 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 12372-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12372-1-AP (IL33 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 12 publications below
• IF: See 3 publications below
• ELISA: See 1 publications below

Product Information

12372-1-AP targets IL-33 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IL-33 fusion protein Ag2957
• Full Name: interleukin 33
• Calculated molecular weight: 30 kDa
• GenBank accession number: BC047085
• Gene symbol: IL33
• Gene ID (NCBI): 90865
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL-33 is an IL-1 family cytokine and nuclear alarmin, is constitutively expressed in epithelial barrier tissues and human blood vessels. IL-33 is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. It also acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury.

Publications

Species	Application	Title
human	IHC,IF	J Invest Dermatol; Increased Type 2 Innate Lymphoid Cells in Patients with Drug Reaction with Eosinophilia and Systemic Symptoms Syndrome.; Authors - Yi-Giien Tsai
rat	WB	Front Immunol; Emodin Alleviates Sodium Taurocholate-Induced Pancreatic Acinar Cell Injury via MicroRNA-30a-5p-Mediated Inhibition of High-Temperature Requirement A/Transforming Growth Factor Beta 1 Inflammatory Signaling.; Authors - Hong Xiang
human	WB	EBioMedicine; Free fatty acid receptor 4 deletion attenuates colitis by modulating Treg Cells via ZBED6-IL33 pathway.; Authors - Shenglong Zhu
mouse	IHC	Exp Dermatol; Topical application of imatinib mesylate suppresses vitamin D3 analog-induced dermatitis in Balb/c mice; Authors - Harutaka Seshimo
mouse	IHC	Front Microbiol; Akkermansia muciniphila Alters Gut Microbiota and Immune System to Improve Cardiovascular Diseases in Murine Model.; Authors - Xin He
mouse	WB	Int Immunopharmacol; Effects of IL-33 on 3T3-L1 cells and obese mice models induced by a high-fat diet.; Authors - Yue Kai