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- KD/KO Validated
IL-33 Polyclonal antibody
IL-33 Polyclonal Antibody for IHC,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 12372-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 12 publications below |
IHC | See 12 publications below |
IF | See 3 publications below |
ELISA | See 1 publications below |
Product Information
12372-1-AP targets IL-33 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | IL-33 fusion protein Ag2957 |
Full Name | interleukin 33 |
Calculated molecular weight | 30 kDa |
GenBank accession number | BC047085 |
Gene symbol | IL33 |
Gene ID (NCBI) | 90865 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IL-33 is an IL-1 family cytokine and nuclear alarmin, is constitutively expressed in epithelial barrier tissues and human blood vessels. IL-33 is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. It also acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury.
Protocols
Product Specific Protocols | |
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WB protocol for IL-33 antibody 12372-1-AP | Download protocol |
IHC protocol for IL-33 antibody 12372-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Invest Dermatol Increased Type 2 Innate Lymphoid Cells in Patients with Drug Reaction with Eosinophilia and Systemic Symptoms Syndrome. | ||
Front Immunol Emodin Alleviates Sodium Taurocholate-Induced Pancreatic Acinar Cell Injury via MicroRNA-30a-5p-Mediated Inhibition of High-Temperature Requirement A/Transforming Growth Factor Beta 1 Inflammatory Signaling. | ||
EBioMedicine Free fatty acid receptor 4 deletion attenuates colitis by modulating Treg Cells via ZBED6-IL33 pathway. | ||
Exp Dermatol Topical application of imatinib mesylate suppresses vitamin D3 analog-induced dermatitis in Balb/c mice | ||
Front Microbiol Akkermansia muciniphila Alters Gut Microbiota and Immune System to Improve Cardiovascular Diseases in Murine Model. | ||
Int Immunopharmacol Effects of IL-33 on 3T3-L1 cells and obese mice models induced by a high-fat diet. |