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PSG1 Monoclonal antibody, PBS Only (Capture) (67269-1-PBS)

Filter: AllWBIHCIF-PCytometric bead array

WB analysis of T-47D using 67269-1-Ig (same clone as 67269-1-PBS)
T-47D cells were subjected to SDS PAGE followed by western blot with 67269-1-Ig (PSG1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67269-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 67269-1-Ig (same clone as 67269-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67269-1-Ig (PSG1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67269-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 67269-1-Ig (same clone as 67269-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67269-1-Ig (PSG1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67269-1-PBS in a different storage buffer formulation.
IF Staining of human placenta using 67269-1-Ig (same clone as 67269-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using PSG1 antibody (67269-1-Ig, Clone: 1C6C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67269-1-PBS in a different storage buffer formulation.
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Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using PSG1 antibody (67269-1-Ig, Clone: 1C6C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67269-1-PBS in a different storage buffer formulation.
IF Staining of human placenta using 67269-1-Ig (same clone as 67269-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using PSG1 antibody (67269-1-Ig, Clone: 1C6C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67269-1-PBS in a different storage buffer formulation.
×
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using PSG1 antibody (67269-1-Ig, Clone: 1C6C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67269-1-PBS in a different storage buffer formulation.
Cytometric bead array standard curve of MP50302-2
Cytometric bead array standard curve of MP50302-2, PSG1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67269-1-PBS. Detection antibody: 67269-4-PBS. Standard:Ag28604. Range: 3.125-100 ng/mL.

PSG1 Monoclonal antibody, PBS Only (Capture)

Name: PSG1 Monoclonal antibody, PBS Only (Capture)
Brand: Proteintech
SKU: 67269-1-PBS

PSG1 Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Cat No. 67269-1-PBS

Clone No.1C6C4

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Synonyms

1C6C4, B1G1, CD66 antigen-like family member F, CD66f, DHFRP2

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

67269-1-PBS targets PSG1 as part of a matched antibody pair:

MP50302-2: 67269-1-PBS capture and 67269-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag28604 Product name: Recombinant human PSG1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 35-349 aa of BC022338 Sequence: QVTIEAEPTKVSEGKDVLLLVHNLPQNLTGYIWYKGQMRDLYHYITSYVVDGEIIIYGPAYSGRETAYSNASLLIQNVTREDAGSYTLHIIKGDDGTRGVTGRFTFTLHLETPKPSISSSNLNPRETMEAVSLTCDPETPDASYLWWMNGQSLPMTHSLKLSETNRTLFLLGVTKYTAGPYECEIRNPVSASRSDPVTLNLLPKLPKPYITINNLNPRENKDVLNFTCEPKSENYTYIWWLNGQSLPVSPRVKRPIENRILILPSVTRNETGPYQCEIRDRYGGIRSDPVTLNVLYGPDLPRIYPSFTYYRSGEV Predict reactive species
Full Name	pregnancy specific beta-1-glycoprotein 1
Calculated Molecular Weight	426 aa, 48 kDa
Observed Molecular Weight	40-70 kDa
GenBank Accession Number	BC022338
Gene Symbol	PSG1
Gene ID (NCBI)	5669
RRID	AB_2882539
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P11464
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Pregnancy-specific β1-glycoproteins (PSGs) are predominant proteins synthesized during gestation in humans, primates and rodents (PMID: 5001318; 11133662). In humans, PSGs are mainly secreted by syncytiotrophoblasts, while in rodents they derive from trophoblast giant cells and spongiotrophoblasts (PMID: 9290143). Moreover, PSGs can serve as indicators for blastocyst quality and embryonic viability (PMID: 8671387; 2409861).