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Neutralization experiment of NeutraKine® IL-10 using 69018-1-Ig (same clone as 69018-1-PBS)
Recombinant human IL-10 (Cat.NO. HZ-1145) stimulates proliferation of MC/9 cells (mouse mast cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human IL-10 (10 ng/mL) is neutralized by mouse anti-human IL-10 monoclonal antibody 69018-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 50-200 ng/mL. This data was developed using the same antibody clone with 69018-1-PBS in a different storage buffer formulation.
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Recombinant human IL-10 (Cat.NO. HZ-1145) stimulates proliferation of MC/9 cells (mouse mast cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human IL-10 (10 ng/mL) is neutralized by mouse anti-human IL-10 monoclonal antibody 69018-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 50-200 ng/mL. This data was developed using the same antibody clone with 69018-1-PBS in a different storage buffer formulation.
Neutralization experiment of NeutraKine® IL-10 using 69018-1-Ig (same clone as 69018-1-PBS)
Recombinant human IL-10 (Cat.NO. HZ-1145) stimulates proliferation of MC/9 cells (mouse mast cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IL-10 (10 ng/mL) is neutralized by mouse anti-human IL-10 monoclonal antibody 69018-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 50-200 ng/mL. The NeutraControl mouse anti-human IL-10 monoclonal antibody 69518-1-Ig could recognize human IL-10 but could not neutralize human IL-10 in the range of 7.8125-2000ng/mL(green curve, refer to top X-right Y axis). This data was developed using the same antibody clone with 69018-1-PBS in a different storage buffer formulation.
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Recombinant human IL-10 (Cat.NO. HZ-1145) stimulates proliferation of MC/9 cells (mouse mast cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IL-10 (10 ng/mL) is neutralized by mouse anti-human IL-10 monoclonal antibody 69018-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 50-200 ng/mL. The NeutraControl mouse anti-human IL-10 monoclonal antibody 69518-1-Ig could recognize human IL-10 but could not neutralize human IL-10 in the range of 7.8125-2000ng/mL(green curve, refer to top X-right Y axis). This data was developed using the same antibody clone with 69018-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 69018-1-Ig (same clone as 69018-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 69018-1-Ig (NeutraKine® IL-10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 69018-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 69018-1-Ig (same clone as 69018-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 69018-1-Ig (NeutraKine® IL-10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 69018-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 69018-1-Ig (same clone as 69018-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 69018-1-Ig (NeutraKine® IL-10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 69018-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 69018-1-Ig (same clone as 69018-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 69018-1-Ig (NeutraKine® IL-10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 69018-1-PBS in a different storage buffer formulation.
ELISA experiment of Recombinant protein using 69018-1-Ig (same clone as 69018-1-PBS)
Indirect ELISA was carried out by coating recombinant Human IL-10 (Cat.NO. HZ-1145) at 70 ng/well followed by blocking and adding serial diluted IL-10 antibody 69018-1-Ig and 69518-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 69018-1-PBS in a different storage buffer formulation.
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Indirect ELISA was carried out by coating recombinant Human IL-10 (Cat.NO. HZ-1145) at 70 ng/well followed by blocking and adding serial diluted IL-10 antibody 69018-1-Ig and 69518-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 69018-1-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 69018-1-Ig
iolayer interferometry (BLl) kinetic assays of 69018-1-Ig against Human IL-10 were performed. The affinity constant is 0.377 nM.

NeutraKine® IL-10 Monoclonal antibody, PBS Only

Name: NeutraKine® IL-10 Monoclonal antibody, PBS Only
Brand: Proteintech
SKU: 69018-1-PBS

NeutraKine® IL-10 Monoclonal Antibody for IHC, Neutralization, ELISA, Indirect ELISA

Cat No. 69018-1-PBS

Clone No.1E4F5

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, Neutralization, ELISA, Indirect ELISA

Synonyms

IL-10, IL-10,IL10, 1E4F5, CSIF, IL 10

Formulation: PBS Only

Sterile PBS

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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Product Information

69018-1-PBS targets NeutraKine® IL-10 in IHC, Neutralization, ELISA, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Product name: HumanKine® recombinant human IL-10 protein Source: -derived, Tag: Domain: of Sequence: Predict reactive species
Full Name	interleukin 10
Gene Symbol	IL-10
Gene ID (NCBI)	3586
RRID	AB_2918838
Conjugate	Unconjugated
Purification Method	Protein G purification
UNIPROT ID	P22301
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

This antibody can be used to neutralize the bioactivity of IL-10.