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  • Immunohistochemistry (IHC) staining of mouse testis tissue using CARHSP1 Recombinant antibody (83300-2-RR)

    IHC staining of mouse testis using 83300-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83300-2-RR (CARHSP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CARHSP1 Recombinant antibody (83300-2-RR)

    IF Staining of HepG2 using 83300-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CARHSP1 antibody (83300-2-RR, Clone: 230489F3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HepG2 cells using CARHSP1 Recombinant antibody (83300-2-RR)

    FC experiment of HepG2 using 83300-2-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CARHSP1 Recombinant antibody (83300-2-RR, Clone:230489F3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83300-2-RR

    Affinity and Kinetic Characterization of 83300-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83300-2-RR against Human CARHSP1 were performed. The affinity constant is 4.36 nM.

CARHSP1 Recombinant antibody

CARHSP1 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83300-2-RR
Clone No.230489F3

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Synonyms

CSDC1, CRHSP-24, CRHSP 24, Calcium-regulated heat-stable protein of 24 kDa, Calcium-regulated heat-stable protein 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83300-2-RR targets CARHSP1 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CARHSP1 fusion protein Ag2265 Predict reactive species
Full Name calcium regulated heat stable protein 1, 24kDa
Calculated Molecular Weight 147 aa, 16 kDa
GenBank Accession NumberBC003366
Gene Symbol CARHSP1
Gene ID (NCBI) 23589
RRIDAB_3670969
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y2V2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for CARHSP1 antibody 83300-2-RRDownload protocol
IF protocol for CARHSP1 antibody 83300-2-RRDownload protocol
FC protocol for CARHSP1 antibody 83300-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols