Tested Applications
| Positive WB detected in | MCF-7 cells, HaCaT cells, SK-BR-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 1 publications below |
Product Information
83345-1-RR targets LYPD3 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag34961 Product name: Recombinant human LYPD3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 31-326 aa of NM_014400 Sequence: LECYSCVQKADDGCSPNKMKTVKCAPGVDVCTEAVGAVETIHGQFSLAVRGCGSGLPGKNDRGLDLHGLLAFIQLQQCAQDRCNAKLNLTSRALDPAGNESAYPPNGVECYSCVGLSREACQGTSPPVVSCYNASDHVYKGCFDGNVTLTAANVTVSLPVRGCVQDEFCTRDGVTGPGFTLSGSCCQGSRCNSDLRNKTYFSPRIPPLVRLPPPEPTTVASTTSVTTSTSAPVRPTSTTKPMPAPTSQTPRQGVEHEASRDEEPRLTGGAAGHQDRSNSGQYPAKGGPQQPHNKGC Predict reactive species |
| Full Name | LY6/PLAUR domain containing 3 |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 75-80 kDa |
| GenBank Accession Number | NM_014400 |
| Gene Symbol | LYPD3 |
| Gene ID (NCBI) | 27076 |
| RRID | AB_3671004 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O95274 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LYPD3 (Ly6/PLAUR domain-containing protein 3), also known as C4.4A , is a membrane protein anchored to the cell surface via a glycosylphosphatidylinositol (GPI) moiety (PMID: 25302166; 15012588). It is a tumorigenic and high-glycosylated protein that has been proven to be linked with the carcinogenic effects in different solid tumors, such as lung cancer and breast cancer (PMID: 32040344; 29371917; 31644911). It has been shown that the elevated expression of LYPD3 is associated with lung adenocarcinoma carcinogenesis and poor prognosis (PMID: 25302166; 32040344).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LYPD3 antibody 83345-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
