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  • Western Blot (WB) analysis of HeLa cells using MOSPD2 Recombinant antibody (83398-1-RR)

    WB analysis of HeLa using 83398-1-RR

    WB result of MOSPD2 antibody (83398-1-RR; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MOSPD2 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using MOSPD2 Recombinant antibody (83398-1-RR)

    WB analysis using 83398-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83398-1-RR (MOSPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MOSPD2 Recombinant antibody (83398-1-RR)

    IHC staining of mouse brain using 83398-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83398-1-RR (MOSPD2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse placenta tissue using MOSPD2 Recombinant antibody (83398-1-RR)

    IHC staining of mouse placenta using 83398-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 83398-1-RR (MOSPD2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of A431 cells using MOSPD2 Recombinant antibody (83398-1-RR)

    FC experiment of A431 using 83398-1-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug MOSPD2 Recombinant antibody (83398-1-RR, Clone:240282C11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83398-1-RR

    Affinity and Kinetic Characterization of 83398-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83398-1 -RR against Human MOSPD2 were performed. The affinity constant is 0.245 nM.

  • Featured Product
  • KD/KO Validated

MOSPD2 Recombinant antibody

MOSPD2 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 83398-1-RR
Clone No.240282C11

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

240282C11, Motile sperm domain-containing protein 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHuH-7 cells, HeLa cells, Jurkat cells, U-937 cells, K-562 cells, RAW 264.7 cells, rat brain tissue
Positive IHC detected inmouse placenta tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83398-1-RR targets MOSPD2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MOSPD2 fusion protein Ag22206 Predict reactive species
Full Name motile sperm domain containing 2
Calculated Molecular Weight 518 aa, 60 kDa
Observed Molecular Weight 59 kDa
GenBank Accession NumberBC030641
Gene Symbol MOSPD2
Gene ID (NCBI) 158747
RRIDAB_3671050
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8NHP6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MOSPD2 is a 518-amino acid protein containing an N-terminal CRAL/TRIO domain, a major sperm protein (MSP) domain, and a potential C-terminal transmembrane domain. MOSPD2 is a member of the vesicle-associated membrane protein-associated protein (VAP) family and one of the endoplasmic reticulum (ER) receptors that function as tether proteins to bridge ER and other organelles to build contacts(PMID: 29858488, 35389430).

Protocols

Product Specific Protocols
WB protocol for MOSPD2 antibody 83398-1-RRDownload protocol
IHC protocol for MOSPD2 antibody 83398-1-RRDownload protocol
FC protocol for MOSPD2 antibody 83398-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols